Potential of Pyridine Amphiphiles as Staphylococcal Nuclease Inhibitor.
Chembiochem
; 19(13): 1400-1408, 2018 07 04.
Article
em En
| MEDLINE
| ID: mdl-29645328
The present study explores the potential of pyridine-based synthetic amphiphiles C1 and C2 having 4-carbon and 12-carbon hydrophobic tails, respectively, as staphylococcal nuclease inhibitors. UV-visible titration with calf-thymus DNA (CT-DNA) revealed a hypochromic shift in the absorbance bands of C1 and C2, whereas fluorescence titration indicated a reduction in the emission intensity of the monomer bands of the amphiphiles. Interaction of deoxyribonucleaseâ
I (DNaseâ
1) and micrococcal nuclease (MNase) with C1 or C2 led to a decrease in the emission intensity of tryptophan at λ=345â
nm along with an increase in the monomer emission intensity of C1 and C2 at λ=375â
nm for DNaseâ
I and excimer emission intensity at λ=470â
nm for both DNaseâ
I and MNase. Scatchard's analysis indicated superior interaction of C2 with DNaseâ
I. Circular dichroism spectroscopy revealed major changes in the secondary structures of both DNaseâ
I and MNase upon interaction with the amphiphiles. A solution-based nuclease assay in conjunction with gel electrophoresis indicated amphiphile-mediated protection against nuclease-directed DNA cleavage. Interestingly, C2 could render inhibition of nuclease present in the culture supernatant of Staphylococcus aureus MTCCâ
96, which highlights the therapeutic prospect of the amphiphile against S.â
aureus.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Piridinas
/
Tensoativos
/
Proteínas de Bactérias
/
Inibidores Enzimáticos
/
Nuclease do Micrococo
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article