Effect of nisin on microbiome-brain-gut axis neurochemicals by Escherichia coli-induced diarrhea in mice.
Microb Pathog
; 119: 65-71, 2018 Jun.
Article
em En
| MEDLINE
| ID: mdl-29649517
ABSTRACT
The effects of nisin on the neurochemicals, Aquaporin-3 (AQP-3) and intestinal microorganisms in the brain-gut axis of mice were analyzed by using enzyme linked immune sorbent assay (ELISA) and high throughput sequencing in this investigation, to further revealed the relationship between intestinal flora abundance in mice and neurochemicals in the brain-gut axis. Using HE staining found damage of structure of small intestine villi in the model group (Escherichia coli O1, E. coli O1). Compared with normal control and ciprofloxacin groups, using ELISA showed that nisin increased the highest norepinephrine (NE) expression in the brain, expression of 5-hydroxytryptamine (5-HT) and dopamine (DA) in the duodenum, and increased the expression of AQP-3 in jejunum. Using high-throughput sequencing showed the highest diversity of cecal microflora in nisin group (ACE-indexâ¯=â¯1417.25, Chao1-indexâ¯=â¯1378.45), but the cecal microflora in the negative control group (ACE-indexâ¯=â¯969.54, Chao1-indexâ¯=â¯340.29) exhibited the lowest species diversity. Our data indicated that nisin regulates neurochemicals, AQP-3 and cecal microflora imbalance in mice.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Diarreia
/
Escherichia coli
/
Microbioma Gastrointestinal
/
Nisina
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article