Estrogen alleviates neuropathic pain induced after spinal cord injury by inhibiting microglia and astrocyte activation.
Biochim Biophys Acta Mol Basis Dis
; 1864(7): 2472-2480, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-29653184
ABSTRACT
Neuropathic pain after spinal cord injury (SCI) is developed in about 80% of SCI patients and there is no efficient therapeutic drug to alleviate SCI-induced neuropathic pain. Here we examined the effect of estrogen on SCI-induced neuropathic pain at below-level and its effect on neuroinflammation as underlying mechanisms. Neuropathic pain is developed at late phase after SCI and a single dose of 17ß-estradiol (100, 300⯵g/kg) were administered to rats with neuropathic pain after SCI through intravenous injection. As results, both mechanical allodynia and thermal hyperalgesia were significantly reduced by 17ß-estradiol compared to vehicle control. Both microglia and astrocyte activation in the lamina I and II of L4-5 dorsal horn was also inhibited by 17ß-estradiol. In addition, the levels of p-p38MAPK and p-ERK known to be activated in microglia and p-JNK known to be activated in astrocyte were significantly decreased by 17ß-estradiol. Furthermore, the mRNA expression of inflammatory mediators such as Il-1ß, Il-6, iNos, and Cox-2 was more attenuated in 17ß-estradiol-treated group than in vehicle-treated group. Particularly, we found that the analgesic effect by 17ß-estradiol was mediated via estrogen receptors, which are expressed in dorsal horn neurons. These results suggest that 17ß-estradiol may attenuate SCI-induced neuropathic pain by inhibiting microglia and astrocyte activation followed inflammation.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Traumatismos da Medula Espinal
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Astrócitos
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Microglia
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Estradiol
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Estrogênios
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Neuralgia
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article