Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures.
Chem Commun (Camb)
; 54(36): 4529-4532, 2018 May 01.
Article
em En
| MEDLINE
| ID: mdl-29662975
Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligodesoxirribonucleotídeos
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DNA
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Engenharia de Proteínas
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Proteínas de Fluorescência Verde
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Nanoestruturas
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article