Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP.

Yang, Yaxiong; Liu, Nan; He, Yuanyuan; Liu, Yuxia; Ge, Lin; Zou, Linzhi; Song, Sen; Xiong, Wei; Liu, Xiaodong

Yang, Yaxiong; Liu, Nan; He, Yuanyuan; Liu, Yuxia; Ge, Lin; Zou, Linzhi; Song, Sen; Xiong, Wei; Liu, Xiaodong.

Afiliação

Yang Y; Department of Biomedical Engineering, School of Medicine, X-Lab for Transmembrane Signaling Research, Tsinghua University, Beijing, 100084, China.
Liu N; School of Biological Science and Medical Engineering, Beihang University, Beijing, 100083, China.
He Y; Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing, 102402, China.
Liu Y; IDG/McGovern Institute for Brain Research, Tsinghua University, Beijing, 100084, China.
Ge L; Department of Biomedical Engineering, School of Medicine, X-Lab for Transmembrane Signaling Research, Tsinghua University, Beijing, 100084, China.
Zou L; School of Life Sciences, Yunan University, Kunming, 650091, China.
Song S; Department of Biomedical Engineering, School of Medicine, X-Lab for Transmembrane Signaling Research, Tsinghua University, Beijing, 100084, China.
Xiong W; Department of Biomedical Engineering, School of Medicine, X-Lab for Transmembrane Signaling Research, Tsinghua University, Beijing, 100084, China.
Liu X; Department of Biomedical Engineering, School of Medicine, X-Lab for Transmembrane Signaling Research, Tsinghua University, Beijing, 100084, China.

Nat Commun ; 9(1): 1504, 2018 04 17.

Article em En | MEDLINE | ID: mdl-29666364

Assuntos

Sinalização do Cálcio; Cálcio/metabolismo; Calmodulina/metabolismo; Corantes Fluorescentes/química; Imagem Molecular/métodos; Animais; Técnicas Biossensoriais/métodos; Cálcio/química; Canais de Cálcio Tipo L/metabolismo; Calmodulina/química; Calmodulina/genética; Engenharia Genética/métodos; Proteínas de Fluorescência Verde/química; Proteínas de Fluorescência Verde/genética; Células HEK293; Humanos; Microscopia Intravital/métodos; Ativação do Canal Iônico; Camundongos; Camundongos Endogâmicos ICR; Neurônios; Técnicas de Patch-Clamp; Cultura Primária de Células; Ligação Proteica

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Calmodulina / Cálcio / Sinalização do Cálcio / Imagem Molecular / Corantes Fluorescentes Limite: Animals / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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