Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late-onset sepsis.

van den Brand, Marre; van den Dungen, Frank A M; Bos, Martine P; van Weissenbruch, Mirjam M; van Furth, A Marceline; de Lange, Annemieke; Rubenjan, Anna; Peters, Remco P H; Savelkoul, Paul H M

van den Brand, Marre; van den Dungen, Frank A M; Bos, Martine P; van Weissenbruch, Mirjam M; van Furth, A Marceline; de Lange, Annemieke; Rubenjan, Anna; Peters, Remco P H; Savelkoul, Paul H M.

Afiliação

van den Brand M; Department of Medical Microbiology and Infection Control, VU University Medical Center, De Boelelaan 1117, 1081HV, Amsterdam, The Netherlands. marre.vandenbrand@vumc.nl.
van den Dungen FAM; Department of Pediatrics, VU University Medical Center, De Boelelaan 1117, 1081HV, Amsterdam, The Netherlands.
Bos MP; Microbiome Ltd., De Boelelaan 1108, 1081HZ, Amsterdam, The Netherlands.
van Weissenbruch MM; Department of Pediatrics, VU University Medical Center, De Boelelaan 1117, 1081HV, Amsterdam, The Netherlands.
van Furth AM; Department of Pediatrics, VU University Medical Center, De Boelelaan 1117, 1081HV, Amsterdam, The Netherlands.
de Lange A; Department of Pediatrics, VU University Medical Center, De Boelelaan 1117, 1081HV, Amsterdam, The Netherlands.
Rubenjan A; Department of Medical Microbiology and Infection Control, VU University Medical Center, De Boelelaan 1117, 1081HV, Amsterdam, The Netherlands.
Peters RPH; Department of Medical Microbiology, University of Pretoria, Lynnwood Road and Roper Street, Hatfield, South Africa.
Savelkoul PHM; Department of Medical Microbiology, Maastricht University Medical Centre+, P. Debyelaan 25, 6229HX, Maastricht, The Netherlands.

Crit Care ; 22(1): 105, 2018 Apr 22.

Article em En | MEDLINE | ID: mdl-29679983

Assuntos

DNA Bacteriano/análise; Sepse/diagnóstico; Carga Bacteriana/imunologia; Carga Bacteriana/métodos; Hemocultura/métodos; Estudos Transversais; DNA Bacteriano/genética; Feminino; Idade Gestacional; Humanos; Recém-Nascido; Recém-Nascido Prematuro/sangue; Recém-Nascido Prematuro/fisiologia; Unidades de Terapia Intensiva Neonatal/organização & administração; Tempo de Internação/estatística & dados numéricos; Masculino; Países Baixos; Reação em Cadeia da Polimerase em Tempo Real/métodos

Palavras-chave

Bacteremia; Bacterial DNA load; Late-onset sepsis; Molecular diagnosis; Neonatology; Real-time PCR

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Bacteriano / Sepse Tipo de estudo: Diagnostic_studies / Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans / Male / Newborn País/Região como assunto: Europa Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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