A signal-on ratiometric fluorometric heparin assay based on the direct interaction between amino-modified carbon dots and DNA.
Mikrochim Acta
; 185(5): 260, 2018 04 21.
Article
em En
| MEDLINE
| ID: mdl-29680977
ABSTRACT
Amino-modified carbon dots (C-dots) with positively charged surface were prepared. They display strong blue fluorescence and are shown to act as quenchers of the green fluorescence of FAM-labeled ssDNA such as the F-probe used in this work that was immobilized on the C-dots. On the addition of highly negatively charged heparin (Hep), it will interact with the C-dots and displace the F-probe from C-dots. Once the F-probe is displaced by Hep, its green fluorescence is restored. The intrinsic blue fluorescence of the C-dots remains stable after addition of Hep. Thus, a signal-on ratiometric fluorometric assay was developed for the ultra-sensitive detection of Hep. The underlying mechanisms of quenching and recovery are discussed. Under optimized conditions, the recovery of the ratiometric fluorescence of the system composed of C-dots and quenched F-probe is proportional to the Hep concentration in the range of 0.01-2.0 µg·mL-1 (= 0.00125-0.25 U·mL-1). The method was successfully applied to the determination of Hep in spiked serum samples. Graphical abstract Schematic of a signal-on ratiometric fluorometric method for the ultra-sensitive detection of heparin on the basis of the displacement and fluorescence enhancement of adsorbed FAM-labeled ssDNA from amino-modified carbon dots (C-dots) by heparin.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bioensaio
/
Carbono
/
Heparina
/
Fluorometria
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article