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Environmental reservoirs for exoS+ and exoU+ strains of Pseudomonas aeruginosa.
Rutherford, Victoria; Yom, Kelly; Ozer, Egon A; Pura, Olivia; Hughes, Ami; Murphy, Katherine R; Cudzilo, Laura; Mitchell, David; Hauser, Alan R.
Afiliação
  • Rutherford V; Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
  • Yom K; Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
  • Ozer EA; Department of Medicine Division of Infectious Diseases, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
  • Pura O; Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
  • Hughes A; Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
  • Murphy KR; Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
  • Cudzilo L; Department of Biology, St. John's University, Collegeville, MN, USA.
  • Mitchell D; Department of Biology, St. John's University, Collegeville, MN, USA.
  • Hauser AR; Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Environ Microbiol Rep ; 10(4): 485-492, 2018 08.
Article em En | MEDLINE | ID: mdl-29687624
Pseudomonas aeruginosa uses its type III secretion system to inject the effector proteins ExoS and ExoU into eukaryotic cells, which subverts these cells to the bacterium's advantage and contributes to severe infections. We studied the environmental reservoirs of exoS+ and exoU+ strains of P. aeruginosa by collecting water, soil, moist substrates and plant samples from environments in the Chicago region and neighbouring states. Whole-genome sequencing was used to determine the phylogeny and type III secretion system genotypes of 120 environmental isolates. No correlation existed between geographic separation of isolates and their genetic relatedness, which confirmed previous findings of both high genetic diversity within a single site and the widespread distribution of P. aeruginosa clonal complexes. After excluding clonal isolates cultured from the same samples, 74 exoS+ isolates and 16 exoU+ isolates remained. Of the exoS+ isolates, 41 (55%) were from natural environmental sites and 33 (45%) were from man-made sites. Of the exoU+ isolates, only 3 (19%) were from natural environmental sites and 13 (81%) were from man-made sites (p < 0.05). These findings suggest that man-made water systems may be a reservoir from which patients acquire exoU+ P. aeruginosa strains.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Proteínas de Bactérias / Toxinas Bacterianas / ADP Ribose Transferases / Microbiologia Ambiental País/Região como assunto: America do norte Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Proteínas de Bactérias / Toxinas Bacterianas / ADP Ribose Transferases / Microbiologia Ambiental País/Região como assunto: America do norte Idioma: En Ano de publicação: 2018 Tipo de documento: Article