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Maternal high fat diet induces early cardiac hypertrophy and alters cardiac metabolism in Sprague Dawley rat offspring.
De Jong, K A; Barrand, S; Wood-Bradley, R J; de Almeida, D L; Czeczor, J K; Lopaschuk, G D; Armitage, J A; McGee, S L.
Afiliação
  • De Jong KA; Metabolic Reprogramming Laboratory, Metabolic Research Unit, School of Medicine, Deakin University, Waurn Ponds, Victoria, Australia. Electronic address: kgraham@deakin.edu.au.
  • Barrand S; Faculty of Health, School of Medicine, Deakin University, Waurn Ponds, Victoria, Australia. Electronic address: s.barrand@deakin.edu.au.
  • Wood-Bradley RJ; Faculty of Health, School of Medicine, Deakin University, Waurn Ponds, Victoria, Australia. Electronic address: r.woodbradley@deakin.edu.au.
  • de Almeida DL; Faculty of Health, School of Medicine, Deakin University, Waurn Ponds, Victoria, Australia. Electronic address: dougalmeida84@gmail.com.
  • Czeczor JK; Institute for Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research, Heinrich-Heine University, c/o Auf'm Hennekamp 65, 40225, Düsseldorf, Germany; German Center of Diabetes Research, Ingolstädter Landstraße 1, 85764, München-Neuherberg, Germany. Electronic address: juju
  • Lopaschuk GD; Mazankowski Alberta Heart Institute, University of Alberta, Edmonton, Canada; Alberta Diabetes Institute, University of Alberta, Edmonton, Canada. Electronic address: gary.lopaschuk@ualberta.ca.
  • Armitage JA; Faculty of Health, School of Medicine, Deakin University, Waurn Ponds, Victoria, Australia. Electronic address: j.armitage@deakin.edu.au.
  • McGee SL; Metabolic Reprogramming Laboratory, Metabolic Research Unit, School of Medicine, Deakin University, Waurn Ponds, Victoria, Australia. Electronic address: sean.mcgee@deakin.edu.au.
Nutr Metab Cardiovasc Dis ; 28(6): 600-609, 2018 06.
Article em En | MEDLINE | ID: mdl-29691147
ABSTRACT
BACKGROUND AND

AIM:

Maternal high fat diets (mHFD) have been associated with an increased offspring cardiovascular risk. Recently we found that the class IIa HDAC-MEF2 pathway regulates gene programs controlling fatty acid oxidation in striated muscle. This same pathway controls hypertrophic responses in the heart. We hypothesized that mHFD is associated with activation of signal controlling class II a HDAC activity and activation of genes involved in fatty acid oxidation and cardiac hypertrophy in offspring. METHODS AND

RESULTS:

Female Sprague Dawley rats were fed either normal fat diet (12%) or high fat diet (43%) three weeks prior to mating, remaining on diets until study completion. Hearts of postnatal day 1 (PN1) and PN10 pups were collected. Bioenergetics and respiration analyses were performed in neonatal ventricular cardiomyocytes (NVCM). In offspring exposed to mHFD, body weight was increased at PN10 accompanied by increased body fat percentage and blood glucose. Heart weight and heart weight to body weight ratio were increased at PN1 and PN10, and were associated with elevated signalling through the AMPK-class IIa HDAC-MEF2 axis. The expression of the MEF2-regulated hypertrophic markers ANP and BNP were increased as were expression of genes involved in fatty acid oxidation. However this was only accompanied by an increased protein expression of fatty acid oxidation enzymes at PN10. NVCM isolated from these pups exhibited increased glycolysis and an impaired substrate flexibility.

CONCLUSION:

Combined, these results suggest that mHFD induces signalling and transcriptional events indicative of reprogrammed cardiac metabolism and of cardiac hypertrophy in Sprague Dawley rat offspring.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Efeitos Tardios da Exposição Pré-Natal / Cardiomegalia / Miócitos Cardíacos / Fenômenos Fisiológicos da Nutrição Materna / Metabolismo Energético / Dieta Hiperlipídica Limite: Animals / Pregnancy Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Efeitos Tardios da Exposição Pré-Natal / Cardiomegalia / Miócitos Cardíacos / Fenômenos Fisiológicos da Nutrição Materna / Metabolismo Energético / Dieta Hiperlipídica Limite: Animals / Pregnancy Idioma: En Ano de publicação: 2018 Tipo de documento: Article