Biosynthesis of D-glucaric acid from sucrose with routed carbon distribution in metabolically engineered Escherichia coli.
Metab Eng
; 47: 393-400, 2018 05.
Article
em En
| MEDLINE
| ID: mdl-29715517
ABSTRACT
D-glucaric acid is a promising platform compound used to synthesize many other value-added or commodity chemicals. The engineering of Escherichia coli for efficiently converting D-glucose to D-glucaric acid has been attempted for several years, with mixed sugar fermentation recently gaining growing interests due to the increased D-glucaric acid yield. Here, we co-expressed cscB, cscA, cscK, ino1, miox, udh, and suhB in E. coli BL21 (DE3), functionally constructing an unreported route from sucrose to D-glucaric acid. Further deletion of chromosomal zwf, pgi, ptsG, uxaC, gudD, over-expression of glk, and use of a D-fructose-dependent translation control system for pgi enabled the strain to use sucrose as the sole carbon source while achieving a high product titer and yield. The titer of D-glucaric acid in M9 medium containing 10â¯g/L sucrose reached ~1.42â¯g/L, with a yield of ~0.142â¯g/g on sucrose.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sacarose
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Escherichia coli
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Engenharia Metabólica
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Microrganismos Geneticamente Modificados
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Ácido Glucárico
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article