Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies-What Does It Mean?
Methods Mol Biol
; 1768: 25-43, 2018.
Article
em En
| MEDLINE
| ID: mdl-29717436
ABSTRACT
Current commercially available digital PCR (dPCR) systems and assays are capable of detecting individual target molecules with considerable reliability. As tests are developed and validated for use on clinical samples, the need to understand and develop robust statistical analysis routines increases. This chapter covers the fundamental processes and limitations of detecting and reporting on single molecule detection. We cover the basics of quantification of targets and sources of imprecision. We describe the basic test concepts sensitivity, specificity, limit of blank, limit of detection, and limit of quantification in the context of dPCR. We provide basic guidelines how to determine those, how to choose and interpret the operating point, and what factors may influence overall test performance in practice.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
Interpretação Estatística de Dados
Tipo de estudo:
Diagnostic_studies
/
Guideline
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article