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Overexpression of 11ß-hydroxysteroid dehydrogenase 1 in visceral adipose tissue and underexpression of endothelial nitric oxide synthase in the adrenal cortex of dogs with hyperadrenocorticism.
Miceli, Diego Daniel; Abiuso, Adriana María Belén; Vidal, Patricia Noemi; Gallelli, María Florencia; Pignataro, Omar Pedro; Castillo, Victor Alejandro.
Afiliação
  • Miceli DD; Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Clínica Médica de Pequeños Animales y Hospital Escuela de Medicina Veterinaria, Unidad de Endocrinología, Av. Chorroarín 280, Buenos Aires CP 1427, Argentina.
  • Abiuso AMB; Laboratorio de Endocrinología Molecular y Transducción de Señales, Instituto de Biología y Medicina Experimental - CONICET, Vuelta de Obligado 2490, Buenos Aires CP 1428, Argentina.
  • Vidal PN; Laboratorio de Endocrinología Molecular y Transducción de Señales, Instituto de Biología y Medicina Experimental - CONICET, Vuelta de Obligado 2490, Buenos Aires CP 1428, Argentina.
  • Gallelli MF; Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Clínica Médica de Pequeños Animales y Hospital Escuela de Medicina Veterinaria, Unidad de Endocrinología, Av. Chorroarín 280, Buenos Aires CP 1427, Argentina.
  • Pignataro OP; Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Clínica Médica de Pequeños Animales y Hospital Escuela de Medicina Veterinaria, Unidad de Endocrinología, Av. Chorroarín 280, Buenos Aires CP 1427, Argentina.
  • Castillo VA; Laboratorio de Endocrinología Molecular y Transducción de Señales, Instituto de Biología y Medicina Experimental - CONICET, Vuelta de Obligado 2490, Buenos Aires CP 1428, Argentina.
Open Vet J ; 8(1): 77-85, 2018.
Article em En | MEDLINE | ID: mdl-29721436
11ß-Hydroxysteroid dehydrogenase 1 (11ß-HSD1) is an enzyme that activates cortisone into cortisol in tissues. Alterations in this enzyme are related to the development of metabolic syndrome, obesity and hyperadrenocorticism (HAC). Endothelial nitric oxide synthase (eNOS) produces nitric oxide and is related to the regulation of adrenal steroidogenesis. The aim of the study was to evaluate 11ß-HSD1 and eNOS expression in dogs with HAC. Visceral adipose tissue samples were taken to evaluate 11ß-HSD1 expression by immunohistochemistry and western blotting. In parallel, adrenal gland samples were collected to evaluate eNOS expression by immunohistochemistry. 11ß-HSD1 expression was significantly higher in the adipocytes of dogs with HAC than in those of the control dogs. eNOS expression in the adrenal cortex (zona fasciculata) was significantly lower in the dogs with HAC than in the control dogs. 11ß-HSD1 overexpression and eNOS underexpression could play a role in the maintenance of hypercortisolism in dogs with HAC.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2018 Tipo de documento: Article