Immune Profiling of T Cells Infiltrating Vitreous Humor in Tubercular Uveitis.
Immunol Invest
; 47(6): 615-631, 2018 Aug.
Article
em En
| MEDLINE
| ID: mdl-29733740
ABSTRACT
PURPOSE:
To assess cellular composition and local cytokine response in vitreous humor of tubercular uveitis.METHODS:
Cells were collected from vitreous cassettes and peripheral blood of 8 tubercular uveitis and 5 control subjects, undergoing vitrectomy and analyzed by flow cytometry for cellular composition, activation status, proinflammatory cytokine expression, and uptake of TLR9 ligand, CpG ODN 2216.RESULTS:
CD3 + T cells with equal proportion of CD4+ and CD8 + T cells formed major fraction of infiltrating cells. The vitreous humor showed higher expression of recent activation marker, CD69, and proinflammatory cytokines, IFN-γ and IL-17A, in CD4 + T cells as compared to peripheral blood. Lastly, intraocular CD4 + T cells showed reduced uptake of ODN 2216 than peripheral blood.CONCLUSIONS:
Our results indicate that local antigenic stimuli trigger T cell infiltration and activation of CD4 + T cells that are hyporesponsive to TLR9 stimulation. These infiltrating T cells might be responsible in further aggravating ocular inflammation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Uveíte
/
Corpo Vítreo
/
Ativação Linfocitária
/
Linfócitos T CD4-Positivos
/
Tuberculose Ocular
/
Linfócitos T CD8-Positivos
Limite:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article