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Exogenous H2 S exerts biphasic effects on porcine mammary epithelial cells proliferation through PI3K/Akt-mTOR signaling pathway.
Zhang, Jing; Ye, Jiayi; Yuan, Cong; Fu, Qin; Zhang, Fenglin; Zhu, Xiaotong; Wang, Lina; Gao, Ping; Shu, Gang; Jiang, Qingyan; Wang, Songbo.
Afiliação
  • Zhang J; Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, P. R. China.
  • Ye J; National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, P. R. China.
  • Yuan C; Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, P. R. China.
  • Fu Q; National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, P. R. China.
  • Zhang F; Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, P. R. China.
  • Zhu X; National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, P. R. China.
  • Wang L; Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, P. R. China.
  • Gao P; National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, P. R. China.
  • Shu G; Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, P. R. China.
  • Jiang Q; National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, P. R. China.
  • Wang S; Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, P. R. China.
J Cell Physiol ; 233(10): 7071-7081, 2018 10.
Article em En | MEDLINE | ID: mdl-29744857
ABSTRACT
This study aimed to investigate the effects of exogenous H2 S on the proliferation of porcine mammary gland epithelial cells (PMECs) and explore the underlying mechanisms. We found that exposure of PMECs to NaHS, at concentrations ranging from 10 to 200 µM, stimulated cell proliferation. However, high concentration of NaHS (600 µM) inhibited PMECs proliferation. Accordingly, 10 µM NaHS significantly increased the percentage of cells undergoing DNA replication, elevated the mRNA and/or protein expression of Cyclin A2, Cyclin D1/3, Cyclin E2 and PCNA, and decreased p21 mRNA expression. In contrast, 600 µM NaHS elicited the opposite effects to that of 10 µM NaHS. In addition, PI3 K/Akt and mTOR signaling pathways were activated or inhibited in response to 10 or 600 µM NaHS, respectively. Furthermore, the promotion of PMECs proliferation, the change of proliferative genes expression, and the activation of mTOR signaling pathway induced by 10 µM NaHS were effectively blocked by PI3 K inhibitor Wortmannin. Similarly, inhibition of mTOR with Rapamycin totally abolished the 10 µM NaHS-induced stimulation of PMECs proliferation and alteration of proliferative genes expression, with no influence on PI3 K/Akt signaling pathway. Moreover, constitutive activation of Akt pathway via transfection of Akt-CA completely eliminated the inhibition of PMECs proliferation and mTOR signaling pathway, and the change of proliferative genes expression induced by 600 µM NaHS. In conclusion, our findings provided evidence that exogenous H2 S supplied by NaHS exerted biphasic effects on PMECs proliferation, with stimulation at lower doses and suppression at high dose, through the intracellular PI3 K/Akt-mTOR signaling pathway.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Apoptose / Proliferação de Células / Células Epiteliais / Sulfeto de Hidrogênio Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Apoptose / Proliferação de Células / Células Epiteliais / Sulfeto de Hidrogênio Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article