[Protective effect of astrocyte exosomes on hypoxic-ischemic neurons].
Zhongguo Dang Dai Er Ke Za Zhi
; 20(5): 397-402, 2018 May.
Article
em Zh
| MEDLINE
| ID: mdl-29764578
ABSTRACT
OBJECTIVE:
To study the effect of astrocyte exosomes on hypoxic-ischemic neurons.METHODS:
Rat astrocytes were cultured in vitro, and differential centrifugation was used to obtain the exosomes from the cell supernatant. Transmission electron microscopy, Nanosight, and Western blot were used for the identification of exosomes. BCA method was used to measure the concentration of exosomes. Rat neurons were cultured in vitro and then divided into control group, exosome group, oxygen glucose deprivation (OGD) group, and OGD+exosome group (n=3 each). The OGD and OGD+exosome groups were cultured in glucose-free medium under the hypoxic condition. The exosome and OGD+exosome groups were treated with exosomes at a final concentration of 22â μg/mL. The control and OGD groups were given an equal volume of phosphate-buffered saline. ELISA was used to measure the level of lactate dehydrogenase (LDH) in neurons. The terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling was used to measure the apoptotic index of neurons.RESULTS:
The identification of exosomes showed that the exosomes extracted by differential centrifugation had the features of exosomes. Compared with the control and exosome groups, the OGD group had significant increases in LDH level and apoptotic index (P<0.05). Compared with the OGD group, the OGD+exosome group had significant reductions in LDH level and apoptotic index (P<0.05).CONCLUSIONS:
The exosomes from astrocytes have a protective effect on neurons with hypoxic-ischemic injury.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Astrócitos
/
Exossomos
/
Neuroproteção
/
Glucose
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2018
Tipo de documento:
Article