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[The role of autophagy in the invasion and metastasis of the squamous cell carcinoma of head and neck].
Chen, C H; Zhu, G C; Pi, L M; Wei, M; She, L; Tan, H L; Liu, G C; Liu, Y; Zhang, X.
Afiliação
  • Chen CH; Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008, China.
  • Zhu GC; Department of Otolaryngology Head and Neck Surgery, the Second Xiangya Hospital, Central South University.
  • Pi LM; Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008, China.
  • Wei M; Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008, China.
  • She L; Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008, China.
  • Tan HL; Department of Head and Neck Surgery, Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya School of Medicine, Central South University.
  • Liu GC; Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008, China.
  • Liu Y; Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008, China.
  • Zhang X; Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi ; 31(10): 789-793, 2017 May 20.
Article em Zh | MEDLINE | ID: mdl-29771045
ABSTRACT

Objective:

The aim of this study is to explore the effects of autophagy on the metastasis of the Squamous Cell Carcinoma of Head and Neck (SCCHN) via epithelial to mesenchymal transition (EMT) induced by TGF-ß1.

Method:

Establish the EMT model induced by TGF-ß1 in the SCCHN in time/concentration, and the expression of autophagy related protein microtubule associated protein 1 light chain3 (LC3) detected by western blot; Autophagy inhibitor chloroquine (CQ), depressing autophagy, the expression of E-cadherin, cytokeratin, Vimentin and LC3 were examined by Western blot. Wound healing and Transwell invasion assay indicate the effects to metastasis for SCCHN.

Result:

Autophagy was activated within TGF-ß1 induced EMT model in the SCCHN in time/concentration dependently. After autophagy was suppressed, the expression of E-cadherin and cytokeratin increased while vimentin and the capacity of metastasis was reduced compared with control group.

Conclusion:

TGF-ß1 induce EMT and Autophagy in the SCCHN. Autophagy could enhances metastasis in the SCCHN via EMT induced by TGF-ß1.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Autofagia / Carcinoma de Células Escamosas / Biomarcadores Tumorais / Neoplasias de Cabeça e Pescoço / Invasividade Neoplásica Tipo de estudo: Prognostic_studies Limite: Humans Idioma: Zh Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Autofagia / Carcinoma de Células Escamosas / Biomarcadores Tumorais / Neoplasias de Cabeça e Pescoço / Invasividade Neoplásica Tipo de estudo: Prognostic_studies Limite: Humans Idioma: Zh Ano de publicação: 2017 Tipo de documento: Article