Development of DNAzyme-based PCR signal cascade amplification for visual detection of Listeria monocytogenes in food.
Anal Biochem
; 553: 7-11, 2018 07 15.
Article
em En
| MEDLINE
| ID: mdl-29777679
ABSTRACT
Listeria monocytogenes is an important foodborne pathogen, and it can cause severe diseases. Rapid detection of L. monocytogenes is crucial to control this pathogen. A simple and robust strategy based on the cascade of PCR and G-quadruplex DNAzyme catalyzed reaction was used to detect L. monocytogenes. In the presence of hemin and the aptamer formed during PCR, the catalytic horseradish peroxidase-mimicking G-quadruplex DNAzymes allow the colorimetric responses of target DNA from L. monocytogenes. This assay can detect genomic DNA of L. monocytogenes specifically with as low as 50 pg/reaction with the naked eye. Through 20 pork samples assay, visual detection assay had the same results as conventional detection methods, and had a good performance. This is a powerful demonstration of the ability of G-quadruplex DNAzyme to be used for PCR-based assay with significant advantages of high sensitivity, low cost and simple manipulation over existing approaches and offers the opportunity for application in pathogen detection.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
/
Reação em Cadeia da Polimerase
/
DNA Catalítico
/
Microbiologia de Alimentos
/
Listeria monocytogenes
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article