Backbone NMR assignments of HypF-N under conditions generating toxic and non-toxic oligomers.
Biomol NMR Assign
; 12(2): 273-277, 2018 10.
Article
em En
| MEDLINE
| ID: mdl-29786756
ABSTRACT
The HypF protein is involved in the maturation and regulation of hydrogenases. The N-terminal domain of HypF (HypF-N) has served as a key model system to study the pathways of protein amyloid formation and the nature of the toxicity of pre-fibrilar protein oligomers. This domain can aggregate into two forms of oligomers having significantly different toxic effects when added to neuronal cultures. Here, NMR assignments of HypF-N backbone resonances are presented in its native state and under the conditions favouring the formation of toxic and non-toxic oligomers. The analyses of chemical shifts provide insights into the protein conformational state and the possible pathways leading to the formation of different types of oligomers.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Ressonância Magnética Nuclear Biomolecular
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Multimerização Proteica
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article