Encapsulation of Negatively Charged Cargo in MS2 Viral Capsids.

Aanei, Ioana L; Glasgow, Jeff E; Capehart, Stacy L; Francis, Matthew B

Aanei, Ioana L; Glasgow, Jeff E; Capehart, Stacy L; Francis, Matthew B.

Afiliação

Aanei IL; Department of Chemistry, University of California, Berkeley, CA, USA.
Glasgow JE; Materials Sciences Division, Lawrence Berkeley National Laboratories, Berkeley, CA, USA.
Capehart SL; Department of Chemistry, University of California, Berkeley, CA, USA.
Francis MB; Department of Chemistry, University of California, Berkeley, CA, USA.

Methods Mol Biol ; 1776: 303-317, 2018.

Article em En | MEDLINE | ID: mdl-29869251

ABSTRACT

ABSTRACT

Encapsulation into virus-like particles is an efficient way of loading cargo of interest for delivery applications. Here, we describe the encapsulation of proteins with tags comprising anionic amino acids or DNA and gold nanoparticles with negative surface charges inside MS2 bacteriophage capsids to obtain homogeneous nanoparticles with a diameter of 27 nm.

Assuntos

Proteínas do Capsídeo/genética; Levivirus/genética; Nanopartículas Metálicas/química; Biologia Molecular/métodos; Capsídeo/virologia; Ouro/química; Vírus da Hepatite B; Humanos; Levivirus/química

Palavras-chave

Delivery vehicles; Encapsulation; MS2 bacteriophage; Nanoparticles; Self-assembly; Virus-like particles

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Levivirus / Proteínas do Capsídeo / Nanopartículas Metálicas / Biologia Molecular Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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