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PD-L1 assessment in pediatric rhabdomyosarcoma: a pilot study.
Bertolini, Giulia; Bergamaschi, Luca; Ferrari, Andrea; Renne, Salvatore L; Collini, Paola; Gardelli, Cecilia; Barisella, Marta; Centonze, Giovanni; Chiaravalli, Stefano; Paolino, Cinzia; Milione, Massimo; Massimino, Maura; Casanova, Michela; Gasparini, Patrizia.
Afiliação
  • Bertolini G; Department of Research, Tumor Genomics Unit, Genomics Unit, Fondazione IRCCS Istituto Nazionale dei Tumori, via venezian 1, 20133, Milan, Italy.
  • Bergamaschi L; Department of Pediatric Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133, Milan, Italy.
  • Ferrari A; Department of Pediatric Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133, Milan, Italy.
  • Renne SL; Soft tissues and bone, and pediatric pathology unit, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133, Milan, Italy.
  • Collini P; Soft tissues and bone, and pediatric pathology unit, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133, Milan, Italy.
  • Gardelli C; Department of Pediatric Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133, Milan, Italy.
  • Barisella M; Pathology Unit, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
  • Centonze G; Department of Research, Tumor Genomics Unit, Genomics Unit, Fondazione IRCCS Istituto Nazionale dei Tumori, via venezian 1, 20133, Milan, Italy.
  • Chiaravalli S; Clinical Research Lab (CRAB), Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
  • Paolino C; Department of Research, Tumor Genomics Unit, Genomics Unit, Fondazione IRCCS Istituto Nazionale dei Tumori, via venezian 1, 20133, Milan, Italy.
  • Milione M; Unit of Thoracic Surgery, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
  • Massimino M; Clinical Research Lab (CRAB), Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
  • Casanova M; Pathology Unit, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
  • Gasparini P; Department of Research, Tumor Genomics Unit, Genomics Unit, Fondazione IRCCS Istituto Nazionale dei Tumori, via venezian 1, 20133, Milan, Italy.
BMC Cancer ; 18(1): 652, 2018 Jun 13.
Article em En | MEDLINE | ID: mdl-29898687
BACKGROUND: Rhabdomyosarcomas (RMSs) are the most frequent soft tissue sarcoma in children and adolescents, defined by skeletal muscle differentiation and the status of FOXO1 fusions. In pediatric malignancies, in particular RMS, scant and controversial observations are reported about PD-L1 expression as a putative biomarker and few immune checkpoint clinical trials are conducted. METHODS: PD-L1 assessment was evaluated by immunohistochemistry (IHC) utilizing two anti-PDL1 antibodies, in a pilot cohort of 25 RMS. Results were confirmed in primary and commercial RMS cell lines by cytofluorimetric analysis and IHC. RESULTS: PD-L1 expression was detectable, by both anti-PD-L1 antibodies, in the immune contexture of immune cells infiltrating and/or surrounding the tumor, in 15/25 (60%) RMS, while absent expression was observed in neoplastic cells. Flow cytometry analysis and PD-L1 IHC of commercial and primary RMS cell lines confirmed a very small percentage of PD-L1 positive-tumor cells, under the detection limits of conventional IHC. Interestingly, increased PD-L1 expression was observed in the immune contexture of 4 RMS cases post chemotherapy compared to their matched pre-treatment samples. CONCLUSION: Here we identify a peculiar pattern of PD-L1 expression in our RMS series with scanty positive-tumor cells detected by flow cytometry, and recurrent expression in the immune cells surrounding or infiltrating the tumor burden.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rabdomiossarcoma / Neoplasias de Tecidos Moles / Biomarcadores Tumorais / Antígeno B7-H1 Tipo de estudo: Observational_studies Limite: Child / Female / Humans / Male Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rabdomiossarcoma / Neoplasias de Tecidos Moles / Biomarcadores Tumorais / Antígeno B7-H1 Tipo de estudo: Observational_studies Limite: Child / Female / Humans / Male Idioma: En Ano de publicação: 2018 Tipo de documento: Article