Allosterism and signal transfer in DNA.
Nucleic Acids Res
; 46(15): 7554-7565, 2018 09 06.
Article
em En
| MEDLINE
| ID: mdl-29905860
ABSTRACT
We analysed the basic mechanisms of signal transmission in DNA and the origins of the allostery exhibited by systems such as the ternary complex BAMHI-DNA-GRDBD. We found that perturbation information generated by a primary protein binding event travels as a wave to distant regions of DNA following a hopping mechanism. However, such a structural perturbation is transient and does not lead to permanent changes in the DNA geometry and interaction properties at the secondary binding site. The BAMHI-DNA-GRDBD allosteric mechanism does not occur through any traditional models direct (protein-protein), indirect (reorganization of the secondary site) readout or solvent-release. On the contrary, it is generated by a subtle and less common entropy-mediated mechanism, which might have an important role to explain other DNA-mediated cooperative effects.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Conformação Proteica
/
DNA
/
Proteínas
/
Conformação de Ácido Nucleico
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article