Versatile Escherichia coli-Bacillus shuttle vectors derived from runaway replication plasmids related to CloDF13.
Mol Gen Genet
; 199(3): 372-80, 1985.
Article
em En
| MEDLINE
| ID: mdl-2993788
ABSTRACT
Versatile cloning vectors were constructed employing a runaway replication mutant of the bacteriocinogenic plasmid CloDF13. These vectors can, under conditions where protein synthesis is not inhibited, be amplified in Escherichia coli to high levels by elevating the temperature and are therefore useful for the production of large quantities of DNA and protein. Since the constructed shuttle vectors, which harbour at least six unique restriction endonucleases sites, replicate in E. coli, Enterobacter cloacae, Staphylococcus aureus and a variety of Bacilli, they are applicable for the genetic engineering of both gram-negative and gram-positive bacteria.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Bacillus
/
Replicação do DNA
/
Escherichia coli
/
Vetores Genéticos
Idioma:
En
Ano de publicação:
1985
Tipo de documento:
Article