Super-resolution Imaging of Amyloid Structures over Extended Times by Using Transient Binding of Single Thioflavinâ
T Molecules.
Chembiochem
; 19(18): 1944-1948, 2018 09 17.
Article
em En
| MEDLINE
| ID: mdl-29953718
ABSTRACT
Oligomeric amyloid structures are crucial therapeutic targets in Alzheimer's and other amyloid diseases. However, these oligomers are too small to be resolved by standard light microscopy. We have developed a simple and versatile tool to image amyloid structures by using thioflavinâ
T without the need for covalent labeling or immunostaining. The dynamic binding of single dye molecules generates photon bursts that are used for fluorophore localization on a nanometer scale. Thus, photobleaching cannot degrade image quality, allowing for extended observation times. Super-resolution transient amyloid binding microscopy promises to directly image native amyloid by using standard probes and record amyloid dynamics over minutes to days. We imaged amyloid fibrils from multiple polypeptides, oligomeric, and fibrillar structures formed during different stages of amyloid-ß aggregation, as well as the structural remodeling of amyloid-ß fibrils by the compound epi-gallocatechin gallate.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos beta-Amiloides
/
Benzotiazóis
/
Imagem Óptica
/
Agregação Patológica de Proteínas
/
Corantes Fluorescentes
/
Amiloide
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article