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Micro-RNAs involved in cellular proliferation have altered expression profiles in granulosa of young women with diminished ovarian reserve.
Woo, Irene; Christenson, Lane K; Gunewardena, Sumedha; Ingles, Sue Ann; Thomas, Semara; Ahmady, Ali; Chung, Karine; Bendikson, Kristin; Paulson, Richard; McGinnis, Lynda K.
Afiliação
  • Woo I; Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, IRD 534, Los Angeles, CA, 90033, USA.
  • Christenson LK; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA.
  • Gunewardena S; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA.
  • Ingles SA; Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, IRD 534, Los Angeles, CA, 90033, USA.
  • Thomas S; Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, IRD 534, Los Angeles, CA, 90033, USA.
  • Ahmady A; Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, IRD 534, Los Angeles, CA, 90033, USA.
  • Chung K; Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, IRD 534, Los Angeles, CA, 90033, USA.
  • Bendikson K; Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, IRD 534, Los Angeles, CA, 90033, USA.
  • Paulson R; Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, IRD 534, Los Angeles, CA, 90033, USA.
  • McGinnis LK; Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, IRD 534, Los Angeles, CA, 90033, USA. Lynda.McGinnnis@usc.edu.
J Assist Reprod Genet ; 35(10): 1777-1786, 2018 Oct.
Article em En | MEDLINE | ID: mdl-29987422
PURPOSE: The study aims to determine differences in micro-RNA (miRNA) expression in granulosa (GC) and cumulus cells (CC) between young women with diminished ovarian reserve (DOR) or normal ovarian reserve (NOR). Secondary objective was to identify downstream signaling pathways that could ultimately indicate causes of lower developmental competence of oocytes from young women with DOR. METHODS: The method of the study is prospective cohort study. RESULTS: Of the miRNA, 125 are differentially expressed in GC between DOR and NOR. Only nine miRNA were different in CC; therefore, we focused analysis on GC. In DOR GC, miR-100-5p, miR-16-5p, miR-30a-3p, and miR-193a-3p were significantly downregulated, while miR-155-5p, miR-192-5p, miR-128-3p, miR-486-5p, miR130a-3p, miR-92a-3p, miR-17-3p, miR-221-3p, and miR-175p were increased. This pattern predicted higher cell proliferation in the DOR GC. The primary pathways include MAPK, Wnt, and TGFbeta. CONCLUSIONS: The miRNA pattern identified critical functions in cell proliferation and survival associated with DOR. GC in women with DOR seems to respond differently to the LH surge.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças Ovarianas / MicroRNAs / Proliferação de Células / Células do Cúmulo / Reserva Ovariana / Células da Granulosa Tipo de estudo: Observational_studies Limite: Adult / Female / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças Ovarianas / MicroRNAs / Proliferação de Células / Células do Cúmulo / Reserva Ovariana / Células da Granulosa Tipo de estudo: Observational_studies Limite: Adult / Female / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article