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Intestinal Lymphatic Endothelial Cells Produce R-Spondin3.
Ogasawara, Reiki; Hashimoto, Daigo; Kimura, Shunsuke; Hayase, Eiko; Ara, Takahide; Takahashi, Shuichiro; Ohigashi, Hiroyuki; Yoshioka, Kosuke; Tateno, Takahiro; Yokoyama, Emi; Ebata, Ko; Kondo, Takeshi; Sugita, Junichi; Onozawa, Masahiro; Iwanaga, Toshihiko; Teshima, Takanori.
Afiliação
  • Ogasawara R; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan.
  • Hashimoto D; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan. D5hash@pop.med.hokudai.ac.jp.
  • Kimura S; Laboratory of Histology and Cytology, Department of Anatomy, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
  • Hayase E; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan.
  • Ara T; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan.
  • Takahashi S; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan.
  • Ohigashi H; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan.
  • Yoshioka K; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan.
  • Tateno T; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan.
  • Yokoyama E; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan.
  • Ebata K; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan.
  • Kondo T; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan.
  • Sugita J; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan.
  • Onozawa M; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan.
  • Iwanaga T; Laboratory of Histology and Cytology, Department of Anatomy, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
  • Teshima T; Department of Hematology, Hokkaido University Faculty of Medicine and Graduate School of Medicine, Sapporo, Japan. teshima@med.hokudai.ac.jp.
Sci Rep ; 8(1): 10719, 2018 Jul 16.
Article em En | MEDLINE | ID: mdl-30013036
The R-Spondin (R-Spo) family regulates WNT signaling and stimulates the proliferation and differentiation of intestinal stem cells (ISCs). R-Spo plays a critical role in maintaining intestinal homeostasis, but endogenous producers of R-Spo in the intestine remain to be investigated. We found that R-Spo3 was the major R-Spo family member produced in the intestine and it was predominantly produced by CD45-CD90+CD31+ lymphatic endothelial cells (LECs) in the lamina propria of the intestinal mucosa. Transcriptome analysis demonstrated that LECs highly expressed R-Spo receptor, Lgr5, suggesting an autocrine stimulatory loop in LECs. LECs were significantly reduced in number, and their R-Spo3 production was impaired in intestinal graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation. The impaired production of R-Spo3 in the intestine may be a novel mechanism of delayed tissue repair and defective mucosal defense in intestinal GVHD. We demonstrate a novel role of intestinal LECs in producing R-Spondin3 to maintain intestinal homeostasis.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Endotélio Linfático / Trombospondinas / Células Endoteliais / Doença Enxerto-Hospedeiro / Mucosa Intestinal Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Endotélio Linfático / Trombospondinas / Células Endoteliais / Doença Enxerto-Hospedeiro / Mucosa Intestinal Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article