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Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections.
Mlodzianoski, Michael J; Cheng-Hathaway, Paul J; Bemiller, Shane M; McCray, Tyler J; Liu, Sheng; Miller, David A; Lamb, Bruce T; Landreth, Gary E; Huang, Fang.
Afiliação
  • Mlodzianoski MJ; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
  • Cheng-Hathaway PJ; Centre for Dynamic Imaging, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
  • Bemiller SM; Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, USA.
  • McCray TJ; Department of Neurosciences, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
  • Liu S; Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN, USA.
  • Miller DA; Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN, USA.
  • Lamb BT; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
  • Landreth GE; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
  • Huang F; Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN, USA.
Nat Methods ; 15(8): 583-586, 2018 08.
Article em En | MEDLINE | ID: mdl-30013047
ABSTRACT
Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-µm-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-ß filaments in a mouse model of Alzheimer's disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Encéfalo / Imagem Individual de Molécula Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Encéfalo / Imagem Individual de Molécula Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article