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Different lesion distribution in calves orally or intratracheally challenged with Mycobacterium bovis: implications for diagnosis.
Serrano, Miriam; Sevilla, Iker A; Fuertes, Miguel; Geijo, Mariví; Risalde, Maria Ángeles; Ruiz-Fons, Jose Francisco; Gortazar, Christian; Juste, Ramón A; Domínguez, Lucas; Elguezabal, Natalia; Garrido, Joseba M.
Afiliação
  • Serrano M; Animal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Derio, Bizkaia, Spain.
  • Sevilla IA; Animal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Derio, Bizkaia, Spain.
  • Fuertes M; Animal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Derio, Bizkaia, Spain.
  • Geijo M; Animal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Derio, Bizkaia, Spain.
  • Risalde MÁ; SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM), Ciudad Real, Spain.
  • Ruiz-Fons JF; Dpto. de Anatomía y Anatomía Patológica Comparadas, Agrifood Campus of International Excellence (ceia3), Universidad de Córdoba, Córdoba, Spain.
  • Gortazar C; SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM), Ciudad Real, Spain.
  • Juste RA; SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM), Ciudad Real, Spain.
  • Domínguez L; Animal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Derio, Bizkaia, Spain.
  • Elguezabal N; SERIDA, Agri-food Research and Development Regional Service, Villaviciosa, Asturias, Spain.
  • Garrido JM; VISAVET Health Surveillance Centre, Complutense University of Madrid, Madrid, Spain.
Vet Res ; 49(1): 74, 2018 07 27.
Article em En | MEDLINE | ID: mdl-30053884
ABSTRACT
Animal tuberculosis (TB) remains a major problem in some countries despite the existence of control programmes focused mainly on cattle. In this species, aerogenous transmission is accepted as the most frequent infection route, affecting mainly the respiratory system. Under the hypothesis that the oral route could be playing a more relevant role in transmission, diagnosis and disease persistence than previously thought, this study was performed to assess the course of TB infection in cattle and its effects on diagnosis depending on the route of entry of Mycobacterium bovis. Two groups of five calves each were either endotracheally (EC) or orally (OC) challenged. Necropsies were carried out 12 weeks after challenge except for three OC calves slaughtered 8 weeks later. All animals reacted to the tuberculin skin test and the entire EC group was positive to the interferon-gamma release assay (IGRA) 2 weeks after challenge and thereafter. The first positive IGRA results for OC calves (3/5) were recorded 4 weeks after challenge. Group comparison revealed significant differences in lesion and positive culture location and scoring. TB-compatible gross lesions and positive cultures were more frequently found in the thorax (p < 0.001) and lung (p < 0.05) of EC animals, whereas OC animals presented lesions (p = 0.23) and positive cultures (p < 0.05) mainly located in the abdomen. These results indicate that the infection route seems to be a determining factor for both the distribution and the time needed for the development of visible lesions. Our study suggests that confirmation of TB infection in some skin reactor animals can be problematic if current post-mortem examination and diagnostics are not improved.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose Bovina / Mycobacterium bovis Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose Bovina / Mycobacterium bovis Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article