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Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV).
Martinez Cuesta, Lucia; Nieto Farias, Maria Victoria; Lendez, Pamela Anahi; Barone, Lucas; Pérez, Sandra Elizabeth; Dolcini, Guillermina Laura; Ceriani, Maria Carolina.
Afiliação
  • Martinez Cuesta L; Laboratorio de Virología, Centro de Investigación Veterinaria de Tandil (CIVETAN, CONICET-CICPBA), Facultad de Cs. Veterinarias, UNCPBA, Pinto 399, Tandil, 7000 Pcia. Buenos Aires, Argentina. Electronic address: lmartinez@vet.unicen.edu.ar.
  • Nieto Farias MV; Laboratorio de Virología, Centro de Investigación Veterinaria de Tandil (CIVETAN, CONICET-CICPBA), Facultad de Cs. Veterinarias, UNCPBA, Pinto 399, Tandil, 7000 Pcia. Buenos Aires, Argentina.
  • Lendez PA; Laboratorio de Virología, Centro de Investigación Veterinaria de Tandil (CIVETAN, CONICET-CICPBA), Facultad de Cs. Veterinarias, UNCPBA, Pinto 399, Tandil, 7000 Pcia. Buenos Aires, Argentina.
  • Barone L; Instituto Nacional de Tecnología Agropecuaria, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Instituto de Virología, Nicolás Repetto y Los Reseros s/n, Hurlingham, B1686, Provincia de Buenos Aires, Argentina.
  • Pérez SE; Laboratorio de Virología, Centro de Investigación Veterinaria de Tandil (CIVETAN, CONICET-CICPBA), Facultad de Cs. Veterinarias, UNCPBA, Pinto 399, Tandil, 7000 Pcia. Buenos Aires, Argentina.
  • Dolcini GL; Laboratorio de Virología, Centro de Investigación Veterinaria de Tandil (CIVETAN, CONICET-CICPBA), Facultad de Cs. Veterinarias, UNCPBA, Pinto 399, Tandil, 7000 Pcia. Buenos Aires, Argentina.
  • Ceriani MC; Laboratorio de Virología, Centro de Investigación Veterinaria de Tandil (CIVETAN, CONICET-CICPBA), Facultad de Cs. Veterinarias, UNCPBA, Pinto 399, Tandil, 7000 Pcia. Buenos Aires, Argentina.
Virus Res ; 256: 11-16, 2018 09 02.
Article em En | MEDLINE | ID: mdl-30055215
ABSTRACT
Bovine leukemia virus (BLV) is a retrovirus that affects cattle causing a lymphoproliferative disease. BLV infection has been associated with misbalance of the immune response causing a higher incidence of other infections. Mastitis is one of the most important conditions that affect milk production in cattle. The aim of this study was to stably infect a bovine mammary epithelial cell line (MAC-T). MAC-T cell line was successfully infected with BLV and the infection was confirmed by nested PCR, qPCR, immunocytochemistry, western blot and transmission electron microscopy. This is the first report of a bovine mammary epithelial cell line stably infected with BLV. This new cell line could be used as an in vitro model to study the effect of BLV on the immune response in the mammary gland and the relationship with other agents causing mastitis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus da Leucemia Bovina / Células Epiteliais Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus da Leucemia Bovina / Células Epiteliais Limite: Animals Idioma: En Ano de publicação: 2018 Tipo de documento: Article