Identification and characterization of PDC1, a novel protein involved in the epigenetic cell degeneration Crippled Growth in Podospora anserina.
Mol Microbiol
; 110(4): 499-512, 2018 11.
Article
em En
| MEDLINE
| ID: mdl-30069939
ABSTRACT
The model fungus Podospora anserina exhibits Crippled Growth (CG), a cell degeneration process linked to the spreading of a prion-like hereditary element. Previous work has shown that the PaMpk1 MAP kinase and the PaNox1 NADPH oxidase are key player in setting up CG. Here, we identified PDC1, a new gene that negatively regulates the PaMpk1 pathway, by identifying the gene mutated in the PDC2205 mutant. This mutant exhibits strong CG in conditions where the wild-type does not. PDC1 encodes a small protein conserved in other Pezizomycotina. The protein contains four evolutionary-conserved cysteines, a tryptophan and a histidine; all six amino-acid are essential for function. PDC1 is located in the cytosol and is present in lower amounts in stationary hyphae in accordance with its role as a repressor. Epistasis analyses place PDC1 between PaMpk1 and PaNox1.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Fúngicas
/
Piruvato Descarboxilase
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Proteínas Quinases Ativadas por Mitógeno
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Podospora
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NADPH Oxidase 1
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article