Gene transfer and molecular cloning of the human NGF receptor.
Science
; 232(4749): 518-21, 1986 Apr 25.
Article
em En
| MEDLINE
| ID: mdl-3008331
ABSTRACT
Nerve growth factor (NGF) and its receptor are important in the development of cells derived from the neural crest. Mouse L cell transformants have been generated that stably express the human NGF receptor gene transfer with total human DNA. Affinity cross-linking, metabolic labeling and immunoprecipitation, and equilibrium binding with 125I-labeled NGF revealed that this NGF receptor had the same size and binding characteristics as the receptor from human melanoma cells and rat PC12 cells. The sequences encoding the NGF receptor were molecularly cloned using the human Alu repetitive sequence as a probe. A cosmid clone that contained the human NGF receptor gene allowed efficient transfection and expression of the receptor.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Clonagem Molecular
/
Receptores de Superfície Celular
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1986
Tipo de documento:
Article