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BioBits™ Bright: A fluorescent synthetic biology education kit.
Stark, Jessica C; Huang, Ally; Nguyen, Peter Q; Dubner, Rachel S; Hsu, Karen J; Ferrante, Thomas C; Anderson, Mary; Kanapskyte, Ada; Mucha, Quinn; Packett, Jessica S; Patel, Palak; Patel, Richa; Qaq, Deema; Zondor, Tyler; Burke, Julie; Martinez, Thomas; Miller-Berry, Ashlee; Puppala, Aparna; Reichert, Kara; Schmid, Miriam; Brand, Lance; Hill, Lander R; Chellaswamy, Jemima F; Faheem, Nuhie; Fetherling, Suzanne; Gong, Elissa; Gonzalzles, Eddie Marie; Granito, Teresa; Koritsaris, Jenna; Nguyen, Binh; Ottman, Sujud; Palffy, Christina; Patel, Angela; Skweres, Sheila; Slaton, Adriane; Woods, TaRhonda; Donghia, Nina; Pardee, Keith; Collins, James J; Jewett, Michael C.
Afiliação
  • Stark JC; Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Technological Institute E136, Evanston, IL 60208-3120, USA.
  • Huang A; Chemistry of Life Processes Institute, Northwestern University, 2170 Campus Drive, Evanston, IL 60208-3120, USA.
  • Nguyen PQ; Center for Synthetic Biology, Northwestern University, 2145 Sheridan Road, Technological Institute E136, Evanston, IL 60208-3120, USA.
  • Dubner RS; Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.
  • Hsu KJ; Institute for Medical Engineering and Science, MIT, Cambridge, MA 02139, USA.
  • Ferrante TC; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
  • Anderson M; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
  • Kanapskyte A; School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
  • Mucha Q; Department of Biological Sciences, Northwestern University, 2205 Tech Drive, Hogan Hall 2144, Evanston, IL 60208, USA.
  • Packett JS; Department of Mechanical Engineering, Northwestern University, 2145 Sheridan Road, Technological Institute B224, Evanston, IL 60208-3120, USA.
  • Patel P; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
  • Patel R; Glenbard East High School, 1014 South Main Street, Lombard, IL 60148-3938, USA.
  • Qaq D; Amos Alonzo Stagg High School, 8015 West 111th Street, Palos Hills, IL 60465-2203, USA.
  • Zondor T; Glenbard East High School, 1014 South Main Street, Lombard, IL 60148-3938, USA.
  • Burke J; Amos Alonzo Stagg High School, 8015 West 111th Street, Palos Hills, IL 60465-2203, USA.
  • Martinez T; Glenbard East High School, 1014 South Main Street, Lombard, IL 60148-3938, USA.
  • Miller-Berry A; Glenbard East High School, 1014 South Main Street, Lombard, IL 60148-3938, USA.
  • Puppala A; Glenbard East High School, 1014 South Main Street, Lombard, IL 60148-3938, USA.
  • Reichert K; Glenbard East High School, 1014 South Main Street, Lombard, IL 60148-3938, USA.
  • Schmid M; Grover Cleveland Elementary School, 3121 West Byron Street, Chicago, IL 60618-3403, USA.
  • Brand L; Glenbard East High School, 1014 South Main Street, Lombard, IL 60148-3938, USA.
  • Hill LR; Evanston Township High School, 1600 Dodge Avenue, Evanston, IL 60201-3449, USA.
  • Chellaswamy JF; Glenbrook South High School, 4000 West Lake Avenue, Glenview, IL 60026-1239, USA.
  • Faheem N; Jones College Prep High School, 700 South State Street, Chicago, IL 60605-2109, USA.
  • Fetherling S; Gwendolyn Brooks College Preparatory Academy, 250 East 111th Street, Chicago, IL 60628-4324, USA.
  • Gong E; Delta High School, 3400 East SR 28, Muncie, IN 47303, USA.
  • Gonzalzles EM; ASPIRA Business and Finance High School, 2989 North Milwaukee Avenue, Chicago, IL 60618-7347, USA.
  • Granito T; Aptakisic-Tripp School District 102, 850 Highland Grove Drive, Buffalo Grove, IL 60089, USA.
  • Koritsaris J; Oak Lawn Hometown Middle School, 5345 West 99th Street, Oak Lawn, IL 60453-3815, USA.
  • Nguyen B; Hoffman Estates High School, 1100 West Higgins Road, Hoffman Estates, IL 60169-4050, USA.
  • Ottman S; Vernon Hills High School, 145 Lakeview Parkway, Vernon Hills, IL 60061-1566, USA.
  • Palffy C; Morgan Park High School, 1744 West Pryor Avenue, Chicago, IL 60643-3497, USA.
  • Patel A; Evanston Township High School, 1600 Dodge Avenue, Evanston, IL 60201-3449, USA.
  • Skweres S; Evanston Township High School, 1600 Dodge Avenue, Evanston, IL 60201-3449, USA.
  • Slaton A; Northside College Prep High School, 5501 North Kedzie Avenue, Chicago, IL 60625-3923, USA.
  • Woods T; Aqsa School, 7361 West 92nd Street, Bridgeview, IL 60455-2133, USA.
  • Donghia N; Adlai E. Stevenson High School, 1 Stevenson Drive, Lincolnshire, IL 60069-2824, USA.
  • Pardee K; Lyons Township High School, 100 South Brainard Avenue, La Grange, IL 60525-2101, USA.
  • Collins JJ; Evanston Township High School, 1600 Dodge Avenue, Evanston, IL 60201-3449, USA.
  • Jewett MC; Evanston Township High School, 1600 Dodge Avenue, Evanston, IL 60201-3449, USA.
Sci Adv ; 4(8): eaat5107, 2018 08.
Article em En | MEDLINE | ID: mdl-30083609
ABSTRACT
Synthetic biology offers opportunities for experiential educational activities at the intersection of the life sciences, engineering, and design. However, implementation of hands-on biology activities in classrooms is challenging because of the need for specialized equipment and expertise to grow living cells. We present BioBits™ Bright, a shelf-stable, just-add-water synthetic biology education kit with easy visual outputs enabled by expression of fluorescent proteins in freeze-dried, cell-free reactions. We introduce activities and supporting curricula for teaching the central dogma, tunable protein expression, and design-build-test cycles and report data generated by K-12 teachers and students. We also develop inexpensive incubators and imagers, resulting in a comprehensive kit costing person classroom. The user-friendly resources of this kit promise to enhance biology education both inside and outside the classroom.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ensino / Técnicas Biossensoriais / Fenômenos Fisiológicos Celulares / Biologia Sintética / Genes Sintéticos / Proteínas Luminescentes Idioma: En Ano de publicação: 2018 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ensino / Técnicas Biossensoriais / Fenômenos Fisiológicos Celulares / Biologia Sintética / Genes Sintéticos / Proteínas Luminescentes Idioma: En Ano de publicação: 2018 Tipo de documento: Article