G1 and S phase arrest in Candida albicans induces filamentous growth via distinct mechanisms.
Mol Microbiol
; 110(2): 191-203, 2018 10.
Article
em En
| MEDLINE
| ID: mdl-30084240
ABSTRACT
Candida albicans is an opportunistic fungal pathogen. In immunocompromised individuals, it can cause bloodstream infections with high mortality rates. The ability to switch between yeast and hyphal morphologies is a critical virulence factor of C. albicans. In response to diverse environmental cues, several signaling pathways are activated resulting in filamentous growth. Interestingly, cell cycle arrest can also trigger filamentous growth although the pathways involved are not well-understood. Here, we demonstrate that the cAMP-PKA pathway is involved in the filamentous growth caused by G1 arrest due to the depletion of the G1 cyclin Cln3 and S phase arrest due to hydroxyurea treatment. The downstream mechanisms involved in filamentation are different between the two cell cycle arrest phenomena. Cln3-depleted cells require HGC1 and UME6 for filamentous growth, but hydroxyurea-induced filamentation does not. Also, the hyphal repressor Nrg1 is not involved in the suppression of Cln3-depletion and hydroxyurea-induced filamentous growth. The findings highlight the complexity of the signaling networks that control filamentous growth in which different mechanisms downstream of the cAMP-PKA pathway are activated based on the nature of the inducing signals.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Candida albicans
/
Proteínas Fúngicas
/
Ciclinas
/
Hifas
/
Pontos de Checagem da Fase G1 do Ciclo Celular
/
Pontos de Checagem da Fase S do Ciclo Celular
Limite:
Humans
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article