Production of d-xylonic acid using a non-recombinant Corynebacterium glutamicum strain.
Bioresour Technol
; 268: 332-339, 2018 Nov.
Article
em En
| MEDLINE
| ID: mdl-30092487
ABSTRACT
It was found that Corynebacterium glutamicum ΔiolR devoid of the transcriptional regulator IolR accumulates high amounts of d-xylonate when cultivated in the presence of d-xylose. Detailed analyses of constructed deletion mutants revealed that the putative myo-inositol 2-dehydrogenase IolG also acts as d-xylose dehydrogenase and is mainly responsible for d-xylonate oxidation in this organism. Process development for d-xylonate production was initiated by cultivating C. glutamicum ΔiolR on defined d-xylose/d-glucose mixtures under batch and fed-batch conditions. The resulting yield matched the theoretical maximum of 1â¯molâ¯mol-1 and high volumetric productivities of up to 4â¯gâ¯L-1â¯h-1 could be achieved. Subsequently, a novel one-pot sequential hydrolysis and fermentation process based on optimized medium containing hydrolyzed sugarcane bagasse was developed. Cost-efficiency and abundance of second-generation substrates, good performance indicators, and enhanced market access using a non-recombinant strain open the perspective for a commercially viable bioprocess for d-xylonate production in the near future.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Xilose
/
Corynebacterium glutamicum
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article