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Genome-scale CRISPR-Cas9 knockout screening in gastrointestinal stromal tumor with Imatinib resistance.
Cao, Jie; Wei, Jianchang; Yang, Ping; Zhang, Tong; Chen, Zhuanpeng; He, Feng; Wei, Fang; Chen, Huacui; Hu, He; Zhong, Junbin; Yang, Zhi; Cai, Wensong; Li, Wanglin; Wang, Qiang.
Afiliação
  • Cao J; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China. doctor_caojie@126.com.
  • Wei J; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China.
  • Yang P; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China.
  • Zhang T; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China.
  • Chen Z; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China.
  • He F; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China.
  • Wei F; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China.
  • Chen H; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China.
  • Hu H; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China.
  • Zhong J; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China.
  • Yang Z; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China.
  • Cai W; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China.
  • Li W; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China. lwl31312008@sina.com.
  • Wang Q; Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, the Second Affiliated Hospital of South China University of Technology, 1 Panfu Road, Guangzhou, 510180, Guangdong, China. wangqiang973@126.com.
Mol Cancer ; 17(1): 121, 2018 08 13.
Article em En | MEDLINE | ID: mdl-30103756
ABSTRACT
Genome-scale CRISPR-Cas9 Knockout Screening was applied to investigate novel targets in imatinib-resistant gastrointestinal stromal tumor (GIST). 20 genes and 2 miRNAs have been selected by total reads of sgRNA and sgRNA diversity, which has been further validated in imatinib-resistant GIST cells by CCK8 and qPCR analysis. Our study has finally revealed 9 genes (DBP, NR3C1, TCF12, TP53, ZNF12, SOCS6, ZFP36, ACYP1, and DRD1) involved in imatinib-resistant GIST-T1 cells. TP53 and SOCS6 may be the most promising candidate genes for imatinib-resistance due to the possible signaling pathway, such as apoptosis pathway and Wnt signaling pathway, JAK-STAT signaling pathway. It is necessary to perform more studies to discover novel targets in imatinib-resistant GIST, including DBP, NR3C1, TCF12, ZNF12, ZFP36, ACYP1 and DRD1.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resistencia a Medicamentos Antineoplásicos / Tumores do Estroma Gastrointestinal / Redes Reguladoras de Genes / Neoplasias Gastrointestinais Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resistencia a Medicamentos Antineoplásicos / Tumores do Estroma Gastrointestinal / Redes Reguladoras de Genes / Neoplasias Gastrointestinais Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article