Your browser doesn't support javascript.
loading
Photothermally Triggered Endosomal Escape and Its Influence on Transfection Efficiency of Gold-Functionalized JetPEI/pDNA Nanoparticles.
Vermeulen, Lotte M P; Fraire, Juan C; Raes, Laurens; De Meester, Ellen; De Keulenaer, Sarah; Van Nieuwerburgh, Filip; De Smedt, Stefaan; Remaut, Katrien; Braeckmans, Kevin.
Afiliação
  • Vermeulen LMP; Laboratory of General Biochemistry & Physical Pharmacy, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. lottem.vermeulen@ugent.be.
  • Fraire JC; Centre for Nano- and Biophotonics, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. lottem.vermeulen@ugent.be.
  • Raes L; Laboratory of General Biochemistry & Physical Pharmacy, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. Juan.Fraire@ugent.be.
  • De Meester E; Centre for Nano- and Biophotonics, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. Juan.Fraire@ugent.be.
  • De Keulenaer S; Laboratory of General Biochemistry & Physical Pharmacy, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. laurens.raes@ugent.be.
  • Van Nieuwerburgh F; Centre for Nano- and Biophotonics, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. laurens.raes@ugent.be.
  • De Smedt S; Laboratory of Pharmaceutical Biotechnology, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. Ellen.Demeester@ugent.be.
  • Remaut K; Laboratory of Pharmaceutical Biotechnology, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. sarah.Dekeulenaer@ugent.be.
  • Braeckmans K; Laboratory of Pharmaceutical Biotechnology, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. filip.Vannieuwerburgh@ugent.be.
Int J Mol Sci ; 19(8)2018 Aug 14.
Article em En | MEDLINE | ID: mdl-30110965
Plasmonic nanoparticles for drug delivery have attracted increasing interest over the last few years. Their localized surface plasmon resonance causes photothermal effects on laser irradiation, which allows for delivering drugs in a spatio-temporally controlled manner. Here, we explore the use of gold nanoparticles (AuNP) as carriers for pDNA in combination with pulsed laser irradiation to induce endosomal escape, which is currently considered to be one of the major bottlenecks in macromolecular drug delivery on the intracellular level. In particular, we evaluate nanocomplexes composed of JetPEI (polyethylenimine)pDNA and 10 nm AuNP, which do not exhibit endosomal escape by themselves. After incubating HeLa cells with these complexes, we evaluated endosomal escape and transfection efficiency using low- and high-energy laser pulses. At low laser energy heat is produced by the nanocomplexes, while, at higher laser energy, explosive vapour nanobubbles (VNB) are formed. We investigated the ability of heat transfer and VNB formation to induce endosomal escape and we examine the integrity of pDNA cargo after inducing both photothermal effects. We conclude that JetPEI/pDNA/AuNP complexes are unable to induce meaningful transfection efficiencies because laser treatment causes either dysfunctionality of the cargo when VNB are formed or forms too small pores in the endosomal membrane to allow pDNA to escape in case of heating. We conclude that laser-induced VNB is the most suitable to induce effective pDNA endosomal escape, but a different nanocomplex structure will be required to keep the pDNA intact.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polietilenoimina / Endossomos / Transfecção / Terapia com Luz de Baixa Intensidade / Nanopartículas Metálicas / Ouro / Hipertermia Induzida / Neoplasias Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polietilenoimina / Endossomos / Transfecção / Terapia com Luz de Baixa Intensidade / Nanopartículas Metálicas / Ouro / Hipertermia Induzida / Neoplasias Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article