Attractiveness of host banana leaf materials to the banana weevil, Cosmopolites sordidus in Ghana for development of field management strategies.

ABSTRACT

BACKGROUND: The banana weevil, Cosmopolites sordidus, has been frequently cited as the most challenging constraint to banana and plantain production, particularly in small-scale (smallholder) farming. For the development of a new, low-cost weevil management technology based on attractive host plant material, we previously identified (2R,5S)-theaspirane as the active component of attractive senesced banana leaves. In this new study, we used behavioural (olfactometer) bioassays with adult weevils to compare the attractiveness of four different developmental stages of banana leaves, i.e. unfolding (pale green), matured green (deep green), matured yellowing and senesced, to determine which leaf developmental stage would be most appropriate for use in weevil management. We also investigated the attractiveness of senesced leaf extracts prepared using different solvents to determine which solvent would be most appropriate for local production of leaf extracts. Coupled gas chromatography-electroantennography (GC-EAG) was then used with adult weevils to confirm the presence of (2R,5S)-theaspirane in attractive leaf extracts. RESULTS: Of the leaf materials tested, only the odour of senesced leaf material was significantly attractive to adult weevils (P < 0.005). Furthermore, an extract of senesced material prepared using palm wine alcohol was significantly attractive (P < 0.05). Using coupled GC-EAG with weevil antennae, (2R,5S)-theaspirane was identified as a minor component with strong EAG activity within the palm wine alcohol extract. CONCLUSION: The results suggest that palm wine alcohol extracts of senesced banana leaf material could be used to lure adult C. sordidus to traps in the field, as part of an ethnobotanical-based approach for C. sordidus management on smallholder farms. © 2018 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.