Identification of a panel of cytokines in neonates with hypoxic ischemic encephalopathy treated with hypothermia.
Cytokine
; 111: 119-124, 2018 11.
Article
em En
| MEDLINE
| ID: mdl-30142532
ABSTRACT
PURPOSE:
Inflammation is a crucial but understudied mechanism of neuronal injury after hypoxia-ischemia. The aim was to identify a panel of cytokines involved in brain injury in neonates with hypoxic ischemic encephalopathy (HIE).METHODS:
Ten newborns with HIE undergoing to therapeutic hypothermia (TH, HIE Group) and 8 healthy newborns (CTRL Group) were enrolled. For the HIE group, 5 samples were collected between 0 and 6â¯h of life (time 1), 12â¯h (time 2), 24â¯h (time 3), 48â¯h (time 4) and 96â¯h of life (time 5). For the CTRL group, one sample was collected. A panel of 48 inflammatory cytokines was determined in all samples. Data were analyzed using multivariate statistical analysis (Principal component analysis, PCA)RESULTS:
17 cytokines, among 48 analyzed, were found to be significantly different, initially, between the CTRL and HIE groups 12 with reported pro-inflammatory effects and 5 with reported anti-inflammatory effects. In the HIE group cytokines showed a decreasing trend during the TH and at the end of treatment comparable to the CTRL group. IL-18 did demonstrate a slight increase at time 3 during HT but decreased steadily at sampling times, 4 and 5.CONCLUSIONS:
Our data demonstrates that many pathways of the inflammatory cascade are activated following hypoxic-ischemic injury. This information will increase our understanding of changes in cytokines over time in neonates with HIE undergoing TH.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Citocinas
/
Hipóxia-Isquemia Encefálica
/
Hipotermia Induzida
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
Limite:
Female
/
Humans
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Male
/
Newborn
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article