Machining protein microcrystals for structure determination by electron diffraction.
Proc Natl Acad Sci U S A
; 115(38): 9569-9573, 2018 09 18.
Article
em En
| MEDLINE
| ID: mdl-30171169
ABSTRACT
We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure determination, bridging the crystal size gap between the nanometer scale of conventional electron diffraction and micron scale of synchrotron microfocus beamlines. The demonstration that atomic information can be retained suggests that milling could provide such detail on sections cut from vitrified cells.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Muramidase
/
Cristalografia por Raios X
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Microtecnologia
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article