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Study of the Potential Radiomitigator Effect of Quercetin on Human Lymphocytes.
de Siqueira, Williams Nascimento; Dos Santos, Felipe Tiago José; de Souza, Thaísa Feliciano; de Vasconcelos Lima, Maíra; Silva, Hianna Arely Milca Fagundes; de Oliveira, Priscilla Stela Santana; da Rocha Pitta, Maira Galdino; Bezerra, Mariana Brayner Cavalcanti Freire; de Salazar E Fernandes, Thiago; de França, Elvis Joacir; da Silva, Edvane Borges; de Albuquerque Melo, Ana Maria Mendonça.
Afiliação
  • de Siqueira WN; Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil. williams.wns@gmail.com.
  • Dos Santos FTJ; Departamento de Energia Nuclear, Universidade Federal de Pernambuco, Recife, Pernambuco, 50740-540, Brazil. williams.wns@gmail.com.
  • de Souza TF; Serviço de Monitoração Ambiental, Centro Regional de Ciências Nucleares do Nordeste, Recife, Pernambuco, 50740-540, Brazil. williams.wns@gmail.com.
  • de Vasconcelos Lima M; Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
  • Silva HAMF; Departamento de Energia Nuclear, Universidade Federal de Pernambuco, Recife, Pernambuco, 50740-540, Brazil.
  • de Oliveira PSS; Departamento de Energia Nuclear, Universidade Federal de Pernambuco, Recife, Pernambuco, 50740-540, Brazil.
  • da Rocha Pitta MG; Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
  • Bezerra MBCF; Serviço de Monitoração Ambiental, Centro Regional de Ciências Nucleares do Nordeste, Recife, Pernambuco, 50740-540, Brazil.
  • de Salazar E Fernandes T; Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
  • de França EJ; Laboratório de Imunomodulação e Novas Abordagens Terapêuticas, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
  • da Silva EB; Laboratório de Imunomodulação e Novas Abordagens Terapêuticas, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
  • de Albuquerque Melo AMM; Departamento de Energia Nuclear, Universidade Federal de Pernambuco, Recife, Pernambuco, 50740-540, Brazil.
Inflammation ; 42(1): 124-134, 2019 Feb.
Article em En | MEDLINE | ID: mdl-30173325
Several substances of synthetic and natural origin have been studied to determine their ability to protect the body from damage caused by ionizing radiation. Among these substances, quercetin has been shown to be a naturally occurring molecule with high radioprotective and radiomitigator potential due to its antioxidant properties. The objective of this work was to ascertain the potential radiomitigator effect of quercetin on chromosome aberration yield in lymphocytes of in vitro-irradiated human peripheral blood. At first, the DPPH (2,2-diphenyl-1-picryl-hydrazyl) radical capture test was performed to determine the antioxidant activity of quercetin and to select the concentrations to be tested. The blood was irradiated at doses of 2.5, 3.5, and 4.5 Gy and lymphocytes were cultured with quercetin at preselected concentrations of 37.5 and 75 µM. Then, the slides were prepared for scoring unstable chromosome aberrations (dicentrics, rings, and fragments). The results showed that the lymphocytes irradiated and later exposed to quercetin presented a lower frequency of chromosomal alterations compared to the control sample which was irradiated and not exposed to quercetin. The results suggest a potential radiomitigator effect of the flavonoid quercetin on human lymphocytes exposed, in vitro, to ionizing radiation. This effect may be related to decrease in the release of cytokines (INF-γ, PGE2, IL-1ß, IL6, IL-8) involved in the proinflammatory processes as well as downregulation of NF-kB and reduction of expression TGF-ß.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quercetina / Protetores contra Radiação / Linfócitos Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quercetina / Protetores contra Radiação / Linfócitos Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article