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Inhibition of Pro-Inflammatory Functions of Human Neutrophils by Constituents of Melodorum fruticosum Leaves.
Engels, Nora S; Waltenberger, Birgit; Michalak, Barbara; Huynh, Loi; Tran, Hung; Kiss, Anna K; Stuppner, Hermann.
Afiliação
  • Engels NS; Institute of Pharmacy/Pharmacognosy and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innrain 80-82, 6020, Innsbruck, Austria.
  • Waltenberger B; Institute of Pharmacy/Pharmacognosy and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innrain 80-82, 6020, Innsbruck, Austria.
  • Michalak B; Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland.
  • Huynh L; Department of Pharmacognosy, Faculty of Pharmacy, University of Medicine and Pharmacy, 41-43 Dinh Tiên Hoàng, Ben Nghé, Quan 1, Ho Chí Minh, Vietnam.
  • Tran H; Department of Pharmacognosy, Faculty of Pharmacy, University of Medicine and Pharmacy, 41-43 Dinh Tiên Hoàng, Ben Nghé, Quan 1, Ho Chí Minh, Vietnam.
  • Kiss AK; Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland.
  • Stuppner H; Institute of Pharmacy/Pharmacognosy and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innrain 80-82, 6020, Innsbruck, Austria.
Chem Biodivers ; 15(11): e1800269, 2018 Nov.
Article em En | MEDLINE | ID: mdl-30193010
In an initial screening, the dichloromethane extract from the leaves of Melodorum fruticosum showed distinct inhibitory effects on the release of interleukin-8 (IL-8) in human neutrophils. Therefore, the aim of the present study was the phytochemical and pharmacological investigation of this extract, to better understand which compounds might be responsible for the anti-inflammatory effect. Phytochemical analysis led to the isolation of 12 known compounds and two new natural products, 5-hydroxy-6-(2-hydroxybenzyl)-4',7-dimethoxyflavanone (13) and 2',4'-dihydroxy-3'-(2-hydroxybenzyl)-4,6'-dimethoxychalcone (14). The influence of the isolated compounds on the production and release of the pro-inflammatory factors IL-8, tumor necrosis factor alpha (TNF-α), reactive oxygen species (ROS), and adhesion molecules (CD62L and CD11b) in human neutrophils was evaluated. Three constituents, melodamide A, 2',4'-dihydroxy-4,6'-dimethoxychalcone, and 2',6'-dihydroxy-4'-methoxychalcone, showed significant inhibition of IL-8 release (IC50 =6.6, 8.6, and 11.6 µm, respectively) and TNF-α production (IC50 =4.5, 13.3, and 6.2 µm, respectively).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anti-Inflamatórios não Esteroides / Annonaceae / Neutrófilos Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anti-Inflamatórios não Esteroides / Annonaceae / Neutrófilos Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article