Replacement of the deletion in the genome (0.762-0.789 mu) of avirulent HSV-1 HFEM using cloned MluI DNA fragment (0.7615-0.796 mu) of virulent HSV-1 F leads to generation of virulent intratypic recombinant.
Virus Res
; 5(2-3): 157-75, 1986 Aug.
Article
em En
| MEDLINE
| ID: mdl-3020829
ABSTRACT
The HFEM strain of HSV-1 is apathogenic for the tree shrew by the intraperitoneal (i.p.) route because of a deletion in the genome coordinates 0.762-0.789. Insertion of the MluI DNA fragment (coordinates 0.7615-0.789) cloned from HSV-1 strain F, which is pathogenic for the tree shrew, restored the i.p. pathogenicity to strain HFEM. The recombinant designated R-M1-C1 was highly pathogenic for the tree shrew, but slightly virulent for inbred mouse strain A. It thus appears that the viral DNA sequence involved in the i.p. pathogenicity of HSV-1 is located within the genome coordinates 0.761-0.796. This sequence is recognized differently by the cellular elements involved in HSV-1 infection in the tree shrew and the mouse.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Deleção Cromossômica
/
Clonagem Molecular
/
Simplexvirus
/
Genes Virais
Limite:
Animals
Idioma:
En
Ano de publicação:
1986
Tipo de documento:
Article