Antioxidant Activity and Neuroprotective Activity of Stilbenoids in Rat Primary Cortex Neurons via the PI3K/Akt Signalling Pathway.
Molecules
; 23(9)2018 Sep 12.
Article
em En
| MEDLINE
| ID: mdl-30213108
ABSTRACT
Antioxidant activity and neuroprotective activity of three stilbenoids, namely, trans-4-hydroxystilbene (THS), trans-3,5,4'-trihydroxy-stilbene (resveratrol, RES), and trans-3',4',3,5-tetrahydroxy-stilbene (piceatannol, PIC), against ß-amyloid (Aß)-induced neurotoxicity in rat primary cortex neurons were evaluated. THS, RES, and PIC significantly scavenged DPPH⢠and â¢OH radicals. All three stilbenoids were able to inhibit Aß neurotoxicity by decreasing intracellular reactive oxygen species (ROS) via the PI3K/Akt signalling pathway. Specifically, stilbenoids significantly promoted Akt phosphorylation; suppressed Bcl-2/Bax expression; and inhibited caspase-9, caspase-3, and PARP cleavage. Molecular docking between stilbenoids with Akt indicated that stilbenoids could form hydrogen bond interactions with the COOH-terminal region of Akt. Additionally, the neuroprotective activity of stilbenoids correlated with the number and position of hydroxyl groups. The lack of meta-dihydroxyl groups on THS did not affect its neuroprotective activity in comparison with RES, whereas the ortho-dihydroxyl moiety on PIC significantly enhanced neuroprotective activity. These results provide new insights into the correlation between the biological activity and chemical structure of stilbenoids.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estilbenos
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Transdução de Sinais
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Córtex Cerebral
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Peptídeos beta-Amiloides
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Fármacos Neuroprotetores
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Neurônios
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Antioxidantes
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article