Residential Proximity to Major Roadways at Birth, DNA Methylation at Birth and Midchildhood, and Childhood Cognitive Test Scores: Project Viva(Massachusetts, USA).
Environ Health Perspect
; 126(9): 97006, 2018 09.
Article
em En
| MEDLINE
| ID: mdl-30226399
ABSTRACT
BACKGROUND:
Epigenetic variability is hypothesized as a regulatory pathway through which prenatal exposures may influence child development and health.OBJECTIVE:
We sought to examine the associations of residential proximity to roadways at birth and epigenome-wide DNA methylation. We also assessed associations of differential methylation with child cognitive outcomes.METHODS:
We estimated residential proximity to roadways at birth using a geographic information system (GIS) and cord blood methylation using Illumina's HumanMethylation450-array in 482 mother-child pairs in Project Viva. We identified individual CpGs associated with residential-proximity-to-roadways at birth using robust linear regression [[Formula see text]]. We also estimated association between proximity-to-roadways at birth and methylation of the same sites in blood samples collected at age 7-11 y ([Formula see text]). We ran the same analyses in the Generation R Study for replication ([Formula see text]). In Project Viva, we investigated associations of differential methylation at birth with midchildhood cognition using linear regression.RESULTS:
Living closer to major roadways at birth was associated with higher cord blood (and-more weakly-midchildhood blood) methylation of four sites in LAMB2. For each halving of residential-proximity-to-major-roadways, we observed a 0.82% increase in DNA methylation at cg05654765 [95% confidence interval (CI) (0.54%, 1.10%)], 0.88% at cg14099457 [95% CI (0.56%, 1.19%)], 0.19% at cg03732535 [95% CI (0.11%, 0.28)], and 1.08% at cg02954987 [95% CI (0.65%, 1.51%)]. Higher cord blood methylation of these sites was associated with lower midchildhood nonverbal cognitive scores. Our results did not replicate in the Generation R Study.CONCLUSIONS:
Our discovery results must be interpreted with caution, given that they were not replicated in a separate cohort. However, living close to major roadways at birth was associated with cord blood methylation of sites in LAMB2-a gene known to be linked to axonal development-in our U.S. cohort. Higher methylation of these sites associated with lower nonverbal cognitive scores at age 7-11 y in the same children. https//doi.org/10.1289/EHP2034.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Efeitos Tardios da Exposição Pré-Natal
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Características de Residência
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Cognição
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Metilação de DNA
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Sangue Fetal
Tipo de estudo:
Etiology_studies
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Incidence_studies
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Observational_studies
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Prognostic_studies
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Risk_factors_studies
Limite:
Child
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Female
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Humans
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Male
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Newborn
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Pregnancy
País/Região como assunto:
America do norte
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article