Mapping the Contact Sites of the <i>Escherichia coli</i> Division-Initiating Proteins FtsZ and ZapA by BAMG Cross-Linking and Site-Directed Mutagenesis.

Roseboom, Winfried; Nazir, Madhvi G; Meiresonne, Nils Y; Mohammadi, Tamimount; Verheul, Jolanda; Buncherd, Hansuk; Bonvin, Alexandre M J J; de Koning, Leo J; de Koster, Chris G; de Jong, Luitzen; den Blaauwen, Tanneke

Mapping the Contact Sites of the Escherichia coli Division-Initiating Proteins FtsZ and ZapA by BAMG Cross-Linking and Site-Directed Mutagenesis.

Roseboom, Winfried; Nazir, Madhvi G; Meiresonne, Nils Y; Mohammadi, Tamimount; Verheul, Jolanda; Buncherd, Hansuk; Bonvin, Alexandre M J J; de Koning, Leo J; de Koster, Chris G; de Jong, Luitzen; den Blaauwen, Tanneke.

Afiliação

Roseboom W; Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. w.roseboom@uva.nl.
Nazir MG; Bacterial Cell Biology and Physiology, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. madhvinazir@gmail.com.
Meiresonne NY; Bacterial Cell Biology and Physiology, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. N.Y.Meiresonne@uva.nl.
Mohammadi T; Bacterial Cell Biology and Physiology, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. tamimohammadi@gmail.com.
Verheul J; Bacterial Cell Biology and Physiology, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. j.verheul@uva.nl.
Buncherd H; Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. hansuk.bu@psu.ac.th.
Bonvin AMJJ; Faculty of Medical Technology, Prince of Songkla University, Songkhla 90110, Thailand. hansuk.bu@psu.ac.th.
de Koning LJ; Computational Structural Biology, Faculty of Science-Chemistry, University of Utrecht, Padualaan 83584CH Utrecht, The Netherlands. a.m.j.j.bonvin@uu.nl.
de Koster CG; Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. L.J.deKoning@uva.nl.
de Jong L; Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. C.G.deKoster@uva.nl.
den Blaauwen T; Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. LuitzendeJong@kpnmail.nl.

Int J Mol Sci ; 19(10)2018 Sep 26.

Article em En | MEDLINE | ID: mdl-30261644

Assuntos

Proteínas de Bactérias/genética; Proteínas de Transporte/genética; Proteínas do Citoesqueleto/genética; Proteínas de Escherichia coli/genética; Escherichia coli/genética; Sequência de Aminoácidos; Proteínas de Bactérias/química; Proteínas de Bactérias/metabolismo; Sítios de Ligação/genética; Proteínas de Transporte/química; Proteínas de Transporte/metabolismo; Divisão Celular/genética; Reagentes de Ligações Cruzadas/química; Proteínas do Citoesqueleto/química; Proteínas do Citoesqueleto/metabolismo; Escherichia coli/metabolismo; Proteínas de Escherichia coli/química; Proteínas de Escherichia coli/metabolismo; Lisina/química; Lisina/genética; Lisina/metabolismo; Espectrometria de Massas/métodos; Simulação de Acoplamento Molecular; Mutagênese Sítio-Dirigida/métodos; Ligação Proteica; Domínios Proteicos; Multimerização Proteica; Software

Palavras-chave

1,4-bis(succimidyl)-3-azidomethylglutarate (BAMG); Filamenting temperature sensitive Z (FtsZ); Fourier-Transform Ion Cyclotron Resonance mass spectrometry(FTICR); Z associated protein A (ZapA); cell division; quadrupole time of flight mass spectrometer (QTOF)

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Proteínas de Transporte / Proteínas de Escherichia coli / Proteínas do Citoesqueleto / Escherichia coli Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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