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Expression of the O-Glycosylation Enzyme GalNAc-T3 in the Equatorial Segment Correlates with the Quality of Spermatozoa.
Nygaard, Marie B; Herlihy, Amy S; Jeanneau, Charlotte; Nielsen, John E; Bennett, Eric Paul; Jørgensen, Niels; Clausen, Henrik; Mandel, Ulla; Rajpert-De Meyts, Ewa; Almstrup, Kristian.
Afiliação
  • Nygaard MB; Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. marie.berg.nygaard@regionh.dk.
  • Herlihy AS; The Fertility Clinic, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. marie.berg.nygaard@regionh.dk.
  • Jeanneau C; Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. menisca@gmail.com.
  • Nielsen JE; Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. charlotte.jeanneau@univ-amu.fr.
  • Bennett EP; Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. JOHN.ERIK.NIELSEN@regionh.dk.
  • Jørgensen N; International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. JOHN.ERIK.NIELSEN@regionh.dk.
  • Clausen H; Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. epb@sund.ku.dk.
  • Mandel U; Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. Niels.Joergensen@regionh.dk.
  • Rajpert-De Meyts E; International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. Niels.Joergensen@regionh.dk.
  • Almstrup K; Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. hclau@sund.ku.dk.
Int J Mol Sci ; 19(10)2018 Sep 27.
Article em En | MEDLINE | ID: mdl-30262754
We question whether the expression of GalNAc-T3, the only known O-GalNAc-transferase present in germ cells, is correlated with qualitative and functional parameters of spermatozoa. We investigated the expression of GalNAc-T3 in ejaculated spermatozoa with immunocytochemistry in swim-up purified and acrosome-reacted spermatozoa from quality-control semen donors and in semen samples from 206 randomly selected men representing a broad spectrum of semen quality. Using donor ejaculates and immunofluorescence detection we found that expression of GalNAc-T3 and the presence of the immature O-glycans Tn and T localized to the equatorial segment of spermatozoa. The proportion of GalNAc-T3-positive spermatozoa in the ejaculate increased after swim-up and appeared unaffected by induction of acrosomal exocytosis. The fraction of spermatozoa with equatorial expression of GalNAc-T3 correlated with classical semen parameters (concentration p = 9 × 10-6, morphology p = 7 × 10-8, and motility p = 1.8 × 10-5) and was significantly lower in men with oligoteratoasthenozoospermia (p = 0.0048). In conclusion, GalNAc-T3 was highly expressed by motile spermatozoa and the expression correlated positively with the classical semen parameters. Therefore, GalNAc-T3 expression seems related to the quality of the spermatozoa, and we propose that reduced expression of GalNAc-T3 may lead to impaired O-glycosylation of proteins and thereby abnormal maturation and reduced functionality of the spermatozoa.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Motilidade dos Espermatozoides / Espermatozoides / N-Acetilgalactosaminiltransferases / Astenozoospermia Tipo de estudo: Qualitative_research Limite: Adult / Humans / Male Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Motilidade dos Espermatozoides / Espermatozoides / N-Acetilgalactosaminiltransferases / Astenozoospermia Tipo de estudo: Qualitative_research Limite: Adult / Humans / Male Idioma: En Ano de publicação: 2018 Tipo de documento: Article