Comparison of ß-Amyloid Plaque Labeling Methods: Antibody Staining, Gallyas Silver Staining, and Thioflavin-S Staining.
Chin Med Sci J
; 33(3): 167-173, 2018 Sep 20.
Article
em En
| MEDLINE
| ID: mdl-30266107
ABSTRACT
Objective To evaluate senile plaque formation and compare the sensitivity of three different ß-amyloid (Aß) labeling methods (antibody staining, Gallyas silver staining, and thioflavin-S staining) to detect Aß deposition.Methods APPswe/PSEN1dE9 transgenic mice (APP/PS1) of different ages were used to examine spatiotemporal changes in Aß plaque deposition. Antibody staining, Gallyas silver staining, and thioflavin-S staining were used to detect Aß plaque deposition in the same brain region of adjacent slices from model mice, and the results were compared.Results With aging, Aß plaques first appeared in the cortex and then the deposition increased throughout the whole brain. Significantly greater plaque deposition was detected by 6E10 antibody than that analyzed with Gallyas silver staining or thioflavin-S staining (P<0.05). Plaque deposition did not show significant difference between the APP/PS1 mice brains assayed with Gallyas silver staining and ones with thioflavin-S staining (P=0.0033).Conclusions The APP/PS1 mouse model of Alzheimer's disease could mimick the progress of Aß plaques occurred in patients with Alzheimer's disease. Antibody detection of Aß deposition may be more sensitive than chemical staining methods.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos beta-Amiloides
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Coloração pela Prata
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Placa Amiloide
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Benzotiazóis
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Anticorpos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article