Beating Vesicles: Encapsulated Protein Oscillations Cause Dynamic Membrane Deformations.
Angew Chem Int Ed Engl
; 57(50): 16286-16290, 2018 12 10.
Article
em En
| MEDLINE
| ID: mdl-30270475
ABSTRACT
The bacterial Min protein system was encapsulated in giant unilamellar vesicles (GUVs). Using confocal fluorescence microscopy, we identified several distinct modes of spatiotemporal patterns inside spherical GUVs. For osmotically deflated GUVs, the vesicle shape actively changed in concert with the Min oscillations. The periodic relocation of Min proteins from the vesicle lumen to the membrane and back is accompanied by drastic changes in the mechanical properties of the lipid bilayer. In particular, two types of oscillating membrane-shape changes are highlighted 1)â
GUVs that repeatedly undergo fission into two connected compartments and fusion of these compartments back into a dumbbell shape and 2)â
GUVs that show periodic budding and subsequent merging of the buds with the mother vesicle, accompanied by an overall shape change of the vesicle reminiscent of a bouncing ball. These findings demonstrate how reaction-diffusion-based protein self-organization can directly yield visible mechanical effects on membrane compartments, even up to autonomous division, without the need for coupling to cytoskeletal elements.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Adenosina Trifosfatases
/
Proteínas de Ciclo Celular
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Proteínas de Escherichia coli
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Escherichia coli
/
Bicamadas Lipídicas
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article