Separation and identification of the epimeric doping agents - Dexamethasone and betamethasone in equine urine and plasma: A reversed phase chiral chromatographic approach.
Steroids
; 140: 77-82, 2018 12.
Article
em En
| MEDLINE
| ID: mdl-30296548
ABSTRACT
Chirality is one of the most important considerations when controlling doping. The epimeric corticosteroids dexamethasone and betamethasone are significantly potent and long-acting, and they are highly abused in equestrian sports. The scope of this study was to develop a simple and reliable analytical method for simultaneously identifying and separating regularly abused co-eluting corticosteroids in equine urine and plasma. In this paper, we present a simple and rapid method for the chiral separation and identification of epimeric mixtures of dexamethasone and betamethasone using a Thermo Q Exactive high resolution accurate mass spectrometer. The high resolution accurate mass spectrometer system provided extremely high sensitivity, enabling detection of each isomer at a very low concentration from complex biological matrices. Chromatographic separation was performed using amylose and cellulose chiral columns. Reversed phase media showed very good potential for providing a successful chiral resolution in LC-MS analysis. This study also focused on optimizing the mobile phase for elution strength, nature of the organic modifier, additives, and column temperature.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise Química do Sangue
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Betametasona
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Dexametasona
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Urinálise
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Cromatografia de Fase Reversa
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article