Your browser doesn't support javascript.
loading
Opposite Regulation of CHOP and GRP78 and Synergistic Apoptosis Induction by Selenium Yeast and Fish Oil via AMPK Activation in Lung Adenocarcinoma Cells.
Kao, Ruey-Ho; Lai, Gi-Ming; Chow, Jyh-Ming; Liao, Chien-Huang; Zheng, Yu-Mei; Tsai, Wei-Lun; Hsia, Simon; Lai, I-Chun; Lee, Hsin-Lun; Chuang, Shuang-En; Whang-Peng, Jacqueline; Yao, Chih-Jung.
Afiliação
  • Kao RH; Cancer Center, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan. rueykao@gmail.com.
  • Lai GM; Division of Hematology and Medical Oncology, Department of Internal Medicine, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan. rueykao@gmail.com.
  • Chow JM; Taipei Cancer Center, Taipei Medical University, Taipei 11031, Taiwan. rueykao@gmail.com.
  • Liao CH; Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan. rueykao@gmail.com.
  • Zheng YM; Cancer Center, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan. gminlai@nhri.org.tw.
  • Tsai WL; Division of Hematology and Medical Oncology, Department of Internal Medicine, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan. gminlai@nhri.org.tw.
  • Hsia S; Taipei Cancer Center, Taipei Medical University, Taipei 11031, Taiwan. gminlai@nhri.org.tw.
  • Lai IC; Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan. gminlai@nhri.org.tw.
  • Lee HL; National Institute of Cancer Research, National Health Research Institutes, Miaoli 35053, Taiwan. gminlai@nhri.org.tw.
  • Chuang SE; Cancer Center, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan. chow0803@yahoo.com.tw.
  • Whang-Peng J; Division of Hematology and Medical Oncology, Department of Internal Medicine, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan. chow0803@yahoo.com.tw.
  • Yao CJ; Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan. chow0803@yahoo.com.tw.
Nutrients ; 10(10)2018 Oct 08.
Article em En | MEDLINE | ID: mdl-30297634
Selenium has been intensively studied for the use of cancer prevention and treatment. However, the clinical effects are still plausible. To enhance its efficacy, a combinational study of selenium yeast (SY) and fish oil (FO) was performed in A549, CL1-0, H1299, HCC827 lung adenocarcinoma (LADC) cells to investigate the enhancement in apoptosis induction and underlying mechanism. By sulforhodamine B staining, Western blot and flow cytometric assays, we found a synergism between SY and FO in growth inhibition and apoptosis induction of LADC cells. In contrast, the fetal lung fibroblast cells (MRC-5) were unsusceptible to this combination effect. FO synergized SY-induced apoptosis of A549 cells, accompanied with synergistic activation of AMP-activated protein kinase (AMPK) and reduction of Cyclooxygenase (COX)-2 and ß-catenin. Particularly, combining with FO not only enhanced the SY-elevated proapoptotic endoplasmic reticulum (ER) stress marker CCAAT/enhancer-binding protein homologous protein (CHOP), but also reduced the cytoprotective glucose regulated protein of molecular weight 78 kDa (GRP78). Consequently, the CHOP downstream targets such as phospho-JNK and death receptor 5 were also elevated, along with the cleavage of caspase-8, -3, and the ER stress-related caspase-4. Accordingly, inhibition of AMPK by compound C diminished the synergistic apoptosis induction, and elevated CHOP/GRP78 ratio by SY combined with FO. The AMPK-dependent synergism suggests the combination of SY and FO for chemoprevention and integrative treatment of LADC.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Selênio / Óleos de Peixe / Adenocarcinoma / Fator de Transcrição CHOP / Proteínas Quinases Ativadas por AMP / Proteínas de Choque Térmico / Neoplasias Pulmonares Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Selênio / Óleos de Peixe / Adenocarcinoma / Fator de Transcrição CHOP / Proteínas Quinases Ativadas por AMP / Proteínas de Choque Térmico / Neoplasias Pulmonares Limite: Humans Idioma: En Ano de publicação: 2018 Tipo de documento: Article