Evaluation of protein-ligand interactions using the luminescent interaction assay FlimPIA with streptavidin-biotin linkage.

Ohmuro-Matsuyama, Yuki; Yamashita, Takahiro; Gomi, Keiko; Yamaji, Hideki; Ueda, Hiroshi

Ohmuro-Matsuyama, Yuki; Yamashita, Takahiro; Gomi, Keiko; Yamaji, Hideki; Ueda, Hiroshi.

Afiliação

Ohmuro-Matsuyama Y; Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, 4259-R1-18 Nagatsuta-cho, Midori-ku, Yokohama, 226-8503, Japan; The Japan Society for the Promotion of Science, 8 Ichiban-Cho, Chiyoda-ku, Tokyo, 102-8472, Japan; Department of Chemical Scienc
Yamashita T; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
Gomi K; Kikkoman Corporation, 250 Noda, Noda, Chiba, 278-8601, Japan.
Yamaji H; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
Ueda H; Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, 4259-R1-18 Nagatsuta-cho, Midori-ku, Yokohama, 226-8503, Japan. Electronic address: ueda@res.titech.ac.jp.

Anal Biochem ; 563: 61-66, 2018 12 15.

Article em En | MEDLINE | ID: mdl-30316750

Assuntos

Biotina/química; Medições Luminescentes/métodos; Estreptavidina/química; Biotinilação; Luciferases de Vaga-Lume/química; Fosforilação; Proteínas Proto-Oncogênicas c-mdm2/química; Proteína Supressora de Tumor p53/química

Palavras-chave

Biotin; Firefly luciferase; Interaction assay; Misfolding; Post-translational modification; Steric hindrance; Streptavidin

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biotina / Estreptavidina / Medições Luminescentes Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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